| Literature DB >> 20224579 |
Claire Rooney1, Gavin White, Alicja Nazgiewicz, Simon A Woodcock, Kurt I Anderson, Christoph Ballestrem, Angeliki Malliri.
Abstract
Focal adhesion (FA) disassembly required for optimal cell migration is mediated by microtubules (MTs); targeting of FAs by MTs coincides with their disassembly. Regrowth of MTs, induced by removal of the MT destabilizer nocodazole, activates the Rho-like GTPase Rac, concomitant with FA disassembly. Here, we show that the Rac guanine nucleotide exchange factor (GEF) Sif and Tiam1-like exchange factor (STEF) is responsible for Rac activation during MT regrowth. Importantly, STEF is required for multiple targeting of FAs by MTs. As a result, FAs in STEF-knockdown cells have a reduced disassembly rate and are consequently enlarged. This leads to reduced speed of migration. Together, these findings suggest a new role for STEF in FA disassembly and cell migration through MT-mediated mechanisms.Entities:
Mesh：
Substances：
Year: 2010 PMID： 20224579 PMCID： PMC2854589 DOI： 10.1038/embor.2010.10
Source DB: PubMed Journal: EMBO Rep ISSN： 1469-221X Impact factor: 8.807