| Literature DB >> 20223085 |
Weiying Yan1, Gary D Byrd, Buddy G Brown, Michael F Borgerding.
Abstract
A new direct method using liquid chromatography-tandem mass spectrometry has been developed and validated for quantitation of 3-hydroxypropylmercapturic acid (3-HPMA) in urine. The method is fast, simple, and does not require extraction from urine. Analyte was separated on a hydrophilic interaction liquid chromatography column. Severe ion suppression was circumvented by a fast gradient after separation. Assay specificity, linearity, precision, and accuracy met the required FDA/CDER bioanalytical method criteria. Matrix effect and carryover of the assay were assessed. Urine sample storage stability and standard solution stability were also tested. The limit of quantitation was 22.0 ng/mL. The results for 3-HPMA obtained by our method were significantly correlated with results obtained by a contract lab.Entities:
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Year: 2010 PMID： 20223085 DOI： 10.1093/chromsci/48.3.194
Source DB: PubMed Journal: J Chromatogr Sci ISSN： 0021-9665 Impact factor: 1.618