PURPOSE: The purpose of this study was to evaluate the effects of cryotop vitrification of sheep cumulus-oocyte complexes (COCs) on oocyte maturation, apoptotic gene expression and incidence of chromosomal abnormalities. METHODS: Freshly isolated (control group) and vitrified-warmed COCs (cryotop group) were matured in vitro. The expression of pro- and anti-apoptotic genes was investigated by real-time PCR. The incidence of numerical chromosomal abnormalities was evaluated by cytogenetic analysis. RESULTS: The mean percentage of oocytes in the cryotop and control groups that reached metaphase II was 49.25 +/- 3.01% and 51.94 +/- 2.7% respectively. The expression rates of pro- and anti-apoptotic genes were similar in both groups, whereas the incidence of numerical chromosomal abnormalities was higher in the cryotop group compared to the control group (42.5% vs. 20%, p < 0.05). CONCLUSION: Although cryotop vitrification of COCs did not affect the incidence of oocyte maturation or apoptotic gene expression, significant deficiencies in the maintenance of oocyte chromosomal organization were seen.
PURPOSE: The purpose of this study was to evaluate the effects of cryotop vitrification of sheep cumulus-oocyte complexes (COCs) on oocyte maturation, apoptotic gene expression and incidence of chromosomal abnormalities. METHODS: Freshly isolated (control group) and vitrified-warmed COCs (cryotop group) were matured in vitro. The expression of pro- and anti-apoptotic genes was investigated by real-time PCR. The incidence of numerical chromosomal abnormalities was evaluated by cytogenetic analysis. RESULTS: The mean percentage of oocytes in the cryotop and control groups that reached metaphase II was 49.25 +/- 3.01% and 51.94 +/- 2.7% respectively. The expression rates of pro- and anti-apoptotic genes were similar in both groups, whereas the incidence of numerical chromosomal abnormalities was higher in the cryotop group compared to the control group (42.5% vs. 20%, p < 0.05). CONCLUSION: Although cryotop vitrification of COCs did not affect the incidence of oocyte maturation or apoptotic gene expression, significant deficiencies in the maintenance of oocyte chromosomal organization were seen.
Authors: J W Mockridge; E C Benton; L V Andreeva; D S Latchman; M S Marber; R J Heads Journal: Biochem Biophys Res Commun Date: 2000-06-24 Impact factor: 3.575
Authors: S Succu; G G Leoni; F Berlinguer; M Madeddu; D Bebbere; F Mossa; L Bogliolo; S Ledda; S Naitana Journal: Theriogenology Date: 2007-05-29 Impact factor: 2.740
Authors: S Succu; G G Leoni; D Bebbere; F Berlinguer; F Mossa; L Bogliolo; M Madeddu; S Ledda; S Naitana Journal: Mol Reprod Dev Date: 2007-10 Impact factor: 2.609
Authors: M Al-Mutary; M Al-Ghadi; A Al-Himaidi; D Iwamoto; Y Al-Anazi; A Ammari; J Ahmad; A Al-Khedhairy Journal: Saudi J Biol Sci Date: 2018-01-31 Impact factor: 4.219
Authors: Z Khodabandeh; I Jamhiri; N Dehghani; H Daneshpazhouh; B Namavar Jahromi; M Dianatpour; S Alaee Journal: Iran J Vet Res Date: 2021 Impact factor: 1.376