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Literature DB >> 20211145

High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis.

Tatiana N Laremore¹, Mellisa Ly, Kemal Solakyildirim, Dmitri V Zagorevski, Robert J Linhardt.

Abstract

Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies. Copyright (c) 2010 Elsevier Inc. All rights reserved.

Entities: CellLine Chemical Disease Species

Mesh：

Substances：
Oligosaccharides
Heparin

Year: 2010 PMID： 20211145 PMCID： PMC2860026 DOI： 10.1016/j.ab.2010.03.004

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

20 in total

Review 1. Electrophoretic approaches to the analysis of complex polysaccharides.

Authors: Nicola Volpi; Francesca Maccari
Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2006-03-10 Impact factor: 3.205

2. Isolation and recovery of acidic oligosaccharides from polyacrylamide gels by semi-dry electrotransfer.

Authors: A al-Hakim; R J Linhardt
Journal: Electrophoresis Date: 1990-01 Impact factor: 3.535

3. Size-exclusion chromatography of heparin oligosaccharides at high and low pressure.

Authors: André Ziegler; Joseph Zaia
Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2006-05-15 Impact factor: 3.205

4. Ethylenediaminetetraacetic acid (EDTA) in heparin.

Authors: E Coyne; H L Messmore; J M Walenga; J Fareed; W H Wehrmacher
Journal: Thromb Res Date: 1998-06-01 Impact factor: 3.944

Review 5. Heparin-protein interactions.

Authors: Ishan Capila; Robert J Linhardt
Journal: Angew Chem Int Ed Engl Date: 2002-02-01 Impact factor: 15.336

6. Fractionation of heparin-derived oligosaccharides by gradient polyacrylamide-gel electrophoresis.

Authors: K G Rice; M K Rottink; R J Linhardt
Journal: Biochem J Date: 1987-06-15 Impact factor: 3.857

7. High-performance liquid chromatographic separation of heparin-derived oligosaccharides.

Authors: K G Rice; Y S Kim; A C Grant; Z M Merchant; R J Linhardt
Journal: Anal Biochem Date: 1985-11-01 Impact factor: 3.365

8. Orthogonal analytical approaches to detect potential contaminants in heparin.

Authors: Marco Guerrini; Zhenqing Zhang; Zachary Shriver; Annamaria Naggi; Sayaka Masuko; Robert Langer; Benito Casu; Robert J Linhardt; Giangiacomo Torri; Ram Sasisekharan
Journal: Proc Natl Acad Sci U S A Date: 2009-09-23 Impact factor: 11.205

9. Metachromatic activity of heparin and heparin fragments.

Authors: A C Grant; R J Linhardt; G L Fitzgerald; J J Park; R Langer
Journal: Anal Biochem Date: 1984-02 Impact factor: 3.365

Review 10. Interactions between heparan sulfate and proteins: the concept of specificity.

Authors: Johan Kreuger; Dorothe Spillmann; Jin-ping Li; Ulf Lindahl
Journal: J Cell Biol Date: 2006-07-31 Impact factor: 10.539

14 in total

1. Asparagine 405 of heparin lyase II prevents the cleavage of glycosidic linkages proximate to a 3-O-sulfoglucosamine residue.

Authors: Wenjing Zhao; Marie-Line Garron; Bo Yang; Zhongping Xiao; Jeffrey D Esko; Miroslaw Cygler; Robert J Linhardt
Journal: FEBS Lett Date: 2011-07-06 Impact factor: 4.124

2. Sequence analysis and domain motifs in the porcine skin decorin glycosaminoglycan chain.

Authors: Xue Zhao; Bo Yang; Kemal Solakyildirim; Kemal Solakylidirim; Eun Ji Joo; Toshihiko Toida; Kyohei Higashi; Robert J Linhardt; Lingyun Li
Journal: J Biol Chem Date: 2013-02-19 Impact factor: 5.157

3. Online Capillary Zone Electrophoresis Negative Electron Transfer Dissociation Tandem Mass Spectrometry of Glycosaminoglycan Mixtures.

Authors: Morgan Stickney; Patience Sanderson; Franklin E Leach; Fuming Zhang; Robert J Linhardt; I Jonathan Amster
Journal: Int J Mass Spectrom Date: 2019-08-17 Impact factor: 1.986

4. Electrospray ionization Fourier transform mass spectrometric analysis of intact bikunin glycosaminoglycan from normal human plasma.

Authors: Tatiana N Laremore; Franklin E Leach; I Jonathan Amster; Robert J Linhardt
Journal: Int J Mass Spectrom Date: 2011-08-15 Impact factor: 1.986

5. Glycosaminoglycan characterization by electrospray ionization mass spectrometry including fourier transform mass spectrometry.

Authors: Tatiana N Laremore; Franklin E Leach; Kemal Solakyildirim; I Jonathan Amster; Robert J Linhardt
Journal: Methods Enzymol Date: 2010 Impact factor: 1.600