Literature DB >> 20210464

Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope.

Yiyan Fei1, James P Landry, Yungshin Sun, Xiangdong Zhu, Xiaobing Wang, Juntao Luo, Chun-Yi Wu, Kit S Lam.   

Abstract

We describe a high-throughput scanning optical microscope for detecting small-molecule compound microarrays on functionalized glass slides. It is based on measurements of oblique-incidence reflectivity difference and employs a combination of a y-scan galvometer mirror and an x-scan translation stage with an effective field of view of 2 cm x 4 cm. Such a field of view can accommodate a printed small-molecule compound microarray with as many as 10,000 to 20,000 targets. The scanning microscope is capable of measuring kinetics as well as endpoints of protein-ligand reactions simultaneously. We present the experimental results on solution-phase protein reactions with small-molecule compound microarrays synthesized from one-bead, one-compound combinatorial chemistry and immobilized on a streptavidin-functionalized glass slide.

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Year:  2010        PMID： 20210464      PMCID： PMC2839802          DOI： 10.1117/1.3309743

Source DB:  PubMed          Journal:  J Biomed Opt        ISSN： 1083-3668            Impact factor:   3.170


  20 in total

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Authors:  Gavin MacBeath
Journal:  Nat Genet       Date:  2002-12       Impact factor: 38.330

2.  Peptide and small molecule microarray for high throughput cell adhesion and functional assays.

Authors:  J R Falsey; M Renil; S Park; S Li; K S Lam
Journal:  Bioconjug Chem       Date:  2001 May-Jun       Impact factor: 4.774

3.  A novel and rapid encoding method based on mass spectrometry for "one-bead-one-compound" small molecule combinatorial libraries.

Authors:  Aimin Song; Jinhua Zhang; Carlito B Lebrilla; Kit S Lam
Journal:  J Am Chem Soc       Date:  2003-05-21       Impact factor: 15.419

4.  A novel approach to chemical microarray using ketone-modified macromolecular scaffolds: application in micro cell-adhesion assay.

Authors:  Qingchai Xu; Suzanne Miyamoto; Kit S Lam
Journal:  Mol Divers       Date:  2004       Impact factor: 2.943

5.  Quantitative methods for spatially resolved adsorption/desorption measurements in real time by surface plasmon resonance microscopy.

Authors:  Jennifer S Shumaker-Parry; Charles T Campbell
Journal:  Anal Chem       Date:  2004-02-15       Impact factor: 6.986

6.  A label-free multisensing immunosensor based on imaging ellipsometry.

Authors:  Zhan Hui Wang; Gang Jin
Journal:  Anal Chem       Date:  2003-11-15       Impact factor: 6.986

7.  A novel approach to protein expression profiling using antibody microarrays combined with surface plasmon resonance technology.

Authors:  Kazue Usui-Aoki; Kiyo Shimada; Mihoko Nagano; Makoto Kawai; Hisashi Koga
Journal:  Proteomics       Date:  2005-06       Impact factor: 3.984

8.  Oblique-incidence reflectivity difference microscope for label-free high-throughput detection of biochemical reactions in a microarray format.

Authors:  Xiangdong Zhu; James P Landry; Yung-Shin Sun; Jeff P Gregg; Kit S Lam; Xiaowen Guo
Journal:  Appl Opt       Date:  2007-04-01       Impact factor: 1.980

9.  Surface plasmon resonance imaging of biomolecular interactions on a grating-based sensor array.

Authors:  Bipin K Singh; Andrew C Hillier
Journal:  Anal Chem       Date:  2006-03-15       Impact factor: 6.986

10.  A new type of synthetic peptide library for identifying ligand-binding activity.

Authors:  K S Lam; S E Salmon; E M Hersh; V J Hruby; W M Kazmierski; R J Knapp
Journal:  Nature       Date:  1991-11-07       Impact factor: 49.962
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  7 in total

1.  Simultaneous measurement of 10,000 protein-ligand affinity constants using microarray-based kinetic constant assays.

Authors:  James P Landry; Yiyan Fei; Xiangdong Zhu
Journal:  Assay Drug Dev Technol       Date:  2011-12-22       Impact factor: 1.738

2.  Fluorescent labeling agents change binding profiles of glycan-binding proteins.

Authors:  Yiyan Fei; Yung-Shin Sun; Yanhong Li; Kam Lau; Hai Yu; Harshal A Chokhawala; Shengshu Huang; James P Landry; Xi Chen; Xiangdong Zhu
Journal:  Mol Biosyst       Date:  2011-10-18

Review 3.  Combinatorial approaches in post-polymerization modification for rational development of therapeutic delivery systems.

Authors:  Yuanbo Zhong; Brian J Zeberl; Xu Wang; Juntao Luo
Journal:  Acta Biomater       Date:  2018-04-12       Impact factor: 8.947

4.  Use of real-time, label-free analysis in revealing low-affinity binding to blood group antigens by Helicobacter pylori.

Authors:  Y Y Fei; A Schmidt; G Bylund; D X Johansson; S Henriksson; C Lebrilla; J V Solnick; T Borén; X D Zhu
Journal:  Anal Chem       Date:  2011-07-19       Impact factor: 6.986

5.  Label-free detection of surface markers on stem cells by oblique-incidence reflectivity difference microscopy.

Authors:  Kai-Yin Lo; Yung-Shin Sun; James P Landry; Xiangdong Zhu; Wenbin Deng
Journal:  Biotechniques       Date:  2011-06       Impact factor: 1.993

6.  An optics-based variable-temperature assay system for characterizing thermodynamics of biomolecular reactions on solid support.

Authors:  Yiyan Fei; James P Landry; Yanhong Li; Hai Yu; Kam Lau; Shengshu Huang; Harshal A Chokhawala; Xi Chen; X D Zhu
Journal:  Rev Sci Instrum       Date:  2013-11       Impact factor: 1.523

7.  Discovering small molecule ligands of vascular endothelial growth factor that block VEGF-KDR binding using label-free microarray-based assays.

Authors:  James P Landry; Yiyan Fei; Xiangdong Zhu; Yaohuang Ke; Guoliang Yu; Pierre Lee
Journal:  Assay Drug Dev Technol       Date:  2013-06       Impact factor: 1.738
  7 in total

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