Literature DB >> 20208521

Chemically ubiquitylated PCNA as a probe for eukaryotic translesion DNA synthesis.

Junjun Chen1, Yongxing Ai, Jialiang Wang, Lajos Haracska, Zhihao Zhuang.   

Abstract

The rapid growth in ubiquitin biology requires facile chemical approaches for protein ubiquitylation that can overcome the common problem of low yield faced by the enzymatic reaction catalyzed by ubiquitin ligases. We report a chemical approach for monoubiquitylation and SUMOylation of PCNA through disulfide exchange and intein chemistry. We used the chemically ubiquitylated and SUMOylated PCNAs in studying translesion DNA synthesis and revealed a surprising degree of flexibility of the ubiquitin modification.

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Year:  2010        PMID： 20208521     DOI： 10.1038/nchembio.316

Source DB:  PubMed          Journal:  Nat Chem Biol        ISSN： 1552-4450            Impact factor:   15.040


  25 in total

1.  Interaction of human DNA polymerase eta with monoubiquitinated PCNA: a possible mechanism for the polymerase switch in response to DNA damage.

Authors:  Patricia L Kannouche; Jonathan Wing; Alan R Lehmann
Journal:  Mol Cell       Date:  2004-05-21       Impact factor: 17.970

2.  A sliding-clamp toolbelt binds high- and low-fidelity DNA polymerases simultaneously.

Authors:  Chiara Indiani; Peter McInerney; Roxana Georgescu; Myron F Goodman; Mike O'Donnell
Journal:  Mol Cell       Date:  2005-09-16       Impact factor: 17.970

Review 3.  PCNA, the maestro of the replication fork.

Authors:  George-Lucian Moldovan; Boris Pfander; Stefan Jentsch
Journal:  Cell       Date:  2007-05-18       Impact factor: 41.582

Review 4.  Regulation of DNA repair throughout the cell cycle.

Authors:  Dana Branzei; Marco Foiani
Journal:  Nat Rev Mol Cell Biol       Date:  2008-02-20       Impact factor: 94.444

Review 5.  Principles of ubiquitin and SUMO modifications in DNA repair.

Authors:  Steven Bergink; Stefan Jentsch
Journal:  Nature       Date:  2009-03-26       Impact factor: 49.962

6.  Regulation of polymerase exchange between Poleta and Poldelta by monoubiquitination of PCNA and the movement of DNA polymerase holoenzyme.

Authors:  Zhihao Zhuang; Robert E Johnson; Lajos Haracska; Louise Prakash; Satya Prakash; Stephen J Benkovic
Journal:  Proc Natl Acad Sci U S A       Date:  2008-04-02       Impact factor: 11.205

7.  Ubiquitylation of yeast proliferating cell nuclear antigen and its implications for translesion DNA synthesis.

Authors:  Lajos Haracska; Ildiko Unk; Louise Prakash; Satya Prakash
Journal:  Proc Natl Acad Sci U S A       Date:  2006-04-12       Impact factor: 11.205

8.  DNA damage tolerance: when it's OK to make mistakes.

Authors:  Debbie J Chang; Karlene A Cimprich
Journal:  Nat Chem Biol       Date:  2009-01-15       Impact factor: 15.040

9.  Control of spontaneous and damage-induced mutagenesis by SUMO and ubiquitin conjugation.

Authors:  Philipp Stelter; Helle D Ulrich
Journal:  Nature       Date:  2003-09-11       Impact factor: 49.962

Review 10.  Translesion synthesis: Y-family polymerases and the polymerase switch.

Authors:  Alan R Lehmann; Atsuko Niimi; Tomoo Ogi; Stephanie Brown; Simone Sabbioneda; Jonathan F Wing; Patricia L Kannouche; Catherine M Green
Journal:  DNA Repair (Amst)       Date:  2007-03-23
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  57 in total

1.  Semisynthetic, site-specific ubiquitin modification of α-synuclein reveals differential effects on aggregation.

Authors:  Franziska Meier; Tharindumala Abeywardana; Abhinav Dhall; Nicholas P Marotta; Jobin Varkey; Ralf Langen; Champak Chatterjee; Matthew R Pratt
Journal:  J Am Chem Soc       Date:  2012-03-14       Impact factor: 15.419

2.  Solution X-ray scattering combined with computational modeling reveals multiple conformations of covalently bound ubiquitin on PCNA.

Authors:  Susan E Tsutakawa; Adam W Van Wynsberghe; Bret D Freudenthal; Christopher P Weinacht; Lokesh Gakhar; M Todd Washington; Zhihao Zhuang; John A Tainer; Ivaylo Ivanov
Journal:  Proc Natl Acad Sci U S A       Date:  2011-10-17       Impact factor: 11.205

Review 3.  Ubiquitin signalling in DNA replication and repair.

Authors:  Helle D Ulrich; Helen Walden
Journal:  Nat Rev Mol Cell Biol       Date:  2010-06-16       Impact factor: 94.444

4.  Damage-specific modification of PCNA.

Authors:  Sapna Das-Bradoo; Hai Dang Nguyen; Anja-Katrin Bielinsky
Journal:  Cell Cycle       Date:  2010-09-21       Impact factor: 4.534

5.  Click chemistry for targeted protein ubiquitylation and ubiquitin chain formation.

Authors:  Daniel Rösner; Tatjana Schneider; Daniel Schneider; Martin Scheffner; Andreas Marx
Journal:  Nat Protoc       Date:  2015-09-24       Impact factor: 13.491

6.  Identification of a functional hotspot on ubiquitin required for stimulation of methyltransferase activity on chromatin.

Authors:  Matthew T Holt; Yael David; Sam Pollock; Zhanyun Tang; Jongcheol Jeon; Jaehoon Kim; Robert G Roeder; Tom W Muir
Journal:  Proc Natl Acad Sci U S A       Date:  2015-08-03       Impact factor: 11.205

7.  Synthesis of a Bis-thio-acetone (BTA) Analogue of the Lysine Isopeptide Bond and its Application to Investigate the Effects of Ubiquitination and SUMOylation on α-Synuclein Aggregation and Toxicity.

Authors:  Yuka E Lewis; Tharindumala Abeywardana; Yu Hsuan Lin; Ana Galesic; Matthew R Pratt
Journal:  ACS Chem Biol       Date:  2016-01-12       Impact factor: 5.100

8.  Intrinsic site-selectivity of ubiquitin dimer formation.

Authors:  Kristen A Andersen; Langdon J Martin; Joel M Prince; Ronald T Raines
Journal:  Protein Sci       Date:  2015-01-15       Impact factor: 6.725

9.  A bacterial genetic selection system for ubiquitylation cascade discovery.

Authors:  Olga Levin-Kravets; Neta Tanner; Noa Shohat; Ilan Attali; Tal Keren-Kaplan; Anna Shusterman; Shay Artzi; Alexander Varvak; Yael Reshef; Xiaojing Shi; Ori Zucker; Tamir Baram; Corine Katina; Inbar Pilzer; Shay Ben-Aroya; Gali Prag
Journal:  Nat Methods       Date:  2016-10-03       Impact factor: 28.547

10.  Site-specific differences in proteasome-dependent degradation of monoubiquitinated α-synuclein.

Authors:  Tharindumala Abeywardana; Yu Hsuan Lin; Ruth Rott; Simone Engelender; Matthew R Pratt
Journal:  Chem Biol       Date:  2013-10-24
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