Literature DB >> 20207863

The SYBR Green I malaria drug sensitivity assay: performance in low parasitemia samples.

Matthias G Vossen1, Sandra Pferschy, Peter Chiba, Harald Noedl.   

Abstract

Validation of the sensitivity of the SYBR Green I in vitro test against an enzyme-linked immunosorbent assay (ELISA)-based drug sensitivity assay. Our results suggest that the SYBR Green I assay is a fast and inexpensive malaria drug screening assay for laboratory use. However, because of its lack of sensitivity in whole blood samples its usefulness for testing clinical samples may be limited.

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Year:  2010        PMID: 20207863      PMCID: PMC2829899          DOI: 10.4269/ajtmh.2010.09-0417

Source DB:  PubMed          Journal:  Am J Trop Med Hyg        ISSN: 0002-9637            Impact factor:   2.345


  11 in total

1.  A novel DNA-based microfluorimetric method to evaluate antimalarial drug activity.

Authors:  Yolanda Corbett; Liuris Herrera; Jose Gonzalez; Luis Cubilla; Todd L Capson; Phyllis D Coley; Thomas A Kursar; Luz I Romero; Eduardo Ortega-Barria
Journal:  Am J Trop Med Hyg       Date:  2004-02       Impact factor: 2.345

2.  In vitro antimalarial activity of azithromycin, artesunate, and quinine in combination and correlation with clinical outcome.

Authors:  Harald Noedl; Srivicha Krudsood; Wattana Leowattana; Noppadon Tangpukdee; Wipa Thanachartwet; Sornchai Looareesuwan; Robert Scott Miller; Mark Fukuda; Krisada Jongsakul; Kritsanai Yingyuen; Sabaithip Sriwichai; Colin Ohrt; Charles Knirsch
Journal:  Antimicrob Agents Chemother       Date:  2006-11-20       Impact factor: 5.191

3.  High-throughput Plasmodium falciparum growth assay for malaria drug discovery.

Authors:  Mary Lynn Baniecki; Dyann F Wirth; Jon Clardy
Journal:  Antimicrob Agents Chemother       Date:  2006-11-20       Impact factor: 5.191

4.  Drug-resistant malaria in Bangui, Central African Republic: an in vitro assessment.

Authors:  Didier Menard; Djibrine Djalle; Alexandre Manirakiza; Ferdinand Yapou; Valerie Siadoua; Serge Sana; Marcelle Diane Matsika-Claquin; Madji Nestor; Antoine Talarmin
Journal:  Am J Trop Med Hyg       Date:  2005-08       Impact factor: 2.345

5.  Comparison of a SYBR green I-based assay with a histidine-rich protein II enzyme-linked immunosorbent assay for in vitro antimalarial drug efficacy testing and application to clinical isolates.

Authors:  David J Bacon; Christine Latour; Carmen Lucas; Olga Colina; Pascal Ringwald; Stéphane Picot
Journal:  Antimicrob Agents Chemother       Date:  2007-01-12       Impact factor: 5.191

6.  Novel, rapid, and inexpensive cell-based quantification of antimalarial drug efficacy.

Authors:  Tyler N Bennett; Michelle Paguio; Bojana Gligorijevic; Clement Seudieu; Andrew D Kosar; Eugene Davidson; Paul D Roepe
Journal:  Antimicrob Agents Chemother       Date:  2004-05       Impact factor: 5.191

7.  Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening.

Authors:  Martin Smilkstein; Nongluk Sriwilaijaroen; Jane Xu Kelly; Prapon Wilairat; Michael Riscoe
Journal:  Antimicrob Agents Chemother       Date:  2004-05       Impact factor: 5.191

8.  Plasmodium falciparum and Plasmodium vivax: lactate dehydrogenase activity and its application for in vitro drug susceptibility assay.

Authors:  L K Basco; F Marquet; M M Makler; J Le Bras
Journal:  Exp Parasitol       Date:  1995-03       Impact factor: 2.011

9.  Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique.

Authors:  R E Desjardins; C J Canfield; J D Haynes; J D Chulay
Journal:  Antimicrob Agents Chemother       Date:  1979-12       Impact factor: 5.191

10.  Performance and reliability of the SYBR Green I based assay for the routine monitoring of susceptibility of Plasmodium falciparum clinical isolates.

Authors:  Marie Ange Rason; Tantely Randriantsoa; Herilalaina Andrianantenaina; Arsène Ratsimbasoa; Didier Menard
Journal:  Trans R Soc Trop Med Hyg       Date:  2008-03-05       Impact factor: 2.184

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  24 in total

1.  Assessment of SYBR green I dye-based fluorescence assay for screening antimalarial activity of cationic peptides and DNA intercalating agents.

Authors:  Rakesh Bhatia; Ankur Gautam; Shailendra K Gautam; Divya Mehta; Vinod Kumar; Gajendra P S Raghava; Grish C Varshney
Journal:  Antimicrob Agents Chemother       Date:  2015-02-17       Impact factor: 5.191

2.  The PfAP2-G2 transcription factor is a critical regulator of gametocyte maturation.

Authors:  Suprita Singh; Joana M Santos; Lindsey M Orchard; Naomi Yamada; Riëtte van Biljon; Heather J Painter; Shaun Mahony; Manuel Llinás
Journal:  Mol Microbiol       Date:  2021-02-15       Impact factor: 3.501

3.  Adaptation and optimization of a fluorescence-based assay for in vivo antimalarial drug screening.

Authors:  Maria H Arias; Eric Deharo; Alexis Valentin; Giovanny Garavito
Journal:  Parasitol Res       Date:  2017-05-16       Impact factor: 2.289

4.  Antimalarial drug sensitivity profile of western Kenya Plasmodium falciparum field isolates determined by a SYBR Green I in vitro assay and molecular analysis.

Authors:  Hoseah M Akala; Fredrick L Eyase; Agnes C Cheruiyot; Angela A Omondi; Bernhards R Ogutu; Norman C Waters; Jacob D Johnson; Mark E Polhemus; David C Schnabel; Douglas S Walsh
Journal:  Am J Trop Med Hyg       Date:  2011-07       Impact factor: 2.345

5.  Validating a firefly luciferase-based high-throughput screening assay for antimalarial drug discovery.

Authors:  Pulin Che; Long Cui; Olaf Kutsch; Liwang Cui; Qianjun Li
Journal:  Assay Drug Dev Technol       Date:  2011-11-03       Impact factor: 1.738

6.  Assessment of the Worldwide Antimalarial Resistance Network Standardized Procedure for In Vitro Malaria Drug Sensitivity Testing Using SYBR Green Assay for Field Samples with Various Initial Parasitemia Levels.

Authors:  Agnes C Cheruiyot; Jennifer M Auschwitz; Patricia J Lee; Redemptah A Yeda; Charles O Okello; Susan E Leed; Mayank Talwar; Tushar Murthy; Heather W Gaona; Mark R Hickman; Hoseah M Akala; Edwin Kamau; Jacob D Johnson
Journal:  Antimicrob Agents Chemother       Date:  2016-03-25       Impact factor: 5.191

7.  Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum.

Authors:  Jeferson Rodrigo Souza Pina; João Victor Silva-Silva; Josiwander Miranda Carvalho; Heriberto Rodrigues Bitencourt; Luciano Almeida Watanabe; Juan Matheus Pereira Fernandes; Guilherme Eduardo de Souza; Anna Caroline Campos Aguiar; Rafael Victorio Carvalho Guido; Fernando Almeida-Souza; Kátia da Silva Calabrese; Patrícia Santana Barbosa Marinho; Andrey Moacir do Rosario Marinho
Journal:  Molecules       Date:  2021-06-02       Impact factor: 4.411

8.  A simple and inexpensive haemozoin-based colorimetric method to evaluate anti-malarial drug activity.

Authors:  Tran Thanh Men; Nguyen Tien Huy; Dai Thi Xuan Trang; Mohammed Nasir Shuaibu; Kenji Hirayama; Kaeko Kamei
Journal:  Malar J       Date:  2012-08-09       Impact factor: 2.979

9.  An image analysis algorithm for malaria parasite stage classification and viability quantification.

Authors:  Seunghyun Moon; Sukjun Lee; Heechang Kim; Lucio H Freitas-Junior; Myungjoo Kang; Lawrence Ayong; Michael A E Hansen
Journal:  PLoS One       Date:  2013-04-23       Impact factor: 3.240

10.  Qualification of standard membrane-feeding assay with Plasmodium falciparum malaria and potential improvements for future assays.

Authors:  Kazutoyo Miura; Bingbing Deng; Gregory Tullo; Ababacar Diouf; Samuel E Moretz; Emily Locke; Merribeth Morin; Michael P Fay; Carole A Long
Journal:  PLoS One       Date:  2013-03-06       Impact factor: 3.240

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