OBJECTIVES: The purpose of the present investigation was to trace the fate of bisphenol A injected into pregnant mice, focusing on its potential accumulation in the fetus and the brain, critical targets of hormonal chemicals, using whole-body autoradiography. METHODS: Pregnant mice were injected intraperitoneally with 0.46MBq of (14)C-BPA and then killed at 1h or 1, 3, or 5 days after injection. Sections for autoradiography were prepared in a cryomicrotome and the exposed imaging plate was processed using a fluorescent/radioisotope image analyzer. RESULTS: Intraperitoneally injected (14)C-BPA was distributed throughout the body, including the fetus and the brain, within 1h. Radioactivity faded gradually from the whole body by the fifth day, and no accumulation in any specific organ was found. However, although (14)C was detected in the fetuses immediately after injection, the transfer of BPA from mother to newborn was not observed. SIGNIFICANCE: The routes of rapid BPA discharge were confirmed, and BPA neither accumulated in the body nor was it transferred to newborn mice. No evidence was observed to suggest the existence of a blood-placenta or blood-brain barrier for BPA. This information should be taken into consideration when assessing the risks of using dental materials that contain BPA. Copyright (c) 2010 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
OBJECTIVES: The purpose of the present investigation was to trace the fate of bisphenol A injected into pregnant mice, focusing on its potential accumulation in the fetus and the brain, critical targets of hormonal chemicals, using whole-body autoradiography. METHODS: Pregnant mice were injected intraperitoneally with 0.46MBq of (14)C-BPA and then killed at 1h or 1, 3, or 5 days after injection. Sections for autoradiography were prepared in a cryomicrotome and the exposed imaging plate was processed using a fluorescent/radioisotope image analyzer. RESULTS: Intraperitoneally injected (14)C-BPA was distributed throughout the body, including the fetus and the brain, within 1h. Radioactivity faded gradually from the whole body by the fifth day, and no accumulation in any specific organ was found. However, although (14)C was detected in the fetuses immediately after injection, the transfer of BPA from mother to newborn was not observed. SIGNIFICANCE: The routes of rapid BPA discharge were confirmed, and BPA neither accumulated in the body nor was it transferred to newborn mice. No evidence was observed to suggest the existence of a blood-placenta or blood-brain barrier for BPA. This information should be taken into consideration when assessing the risks of using dental materials that contain BPA. Copyright (c) 2010 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
Authors: Anabela Baptista Paula; Debbie Toste; Alfredo Marinho; Inês Amaro; Carlos-Miguel Marto; Ana Coelho; Manuel Marques-Ferreira; Eunice Carrilho Journal: Int J Environ Res Public Health Date: 2019-05-09 Impact factor: 3.390