Literature DB >> 20194699

Use of a high-resolution melt assay to characterize codon 54 of the cyp51A gene of Aspergillus fumigatus on a Rotor-Gene 6000 instrument.

M J Tuohy1, V Reja, S Park, D S Perlin, M Wnek, G W Procop, B Yen-Lieberman.   

Abstract

A high-resolution melt (HRM) assay using a Rotor-Gene 6000 instrument was developed to characterize the codon for glycine 54 in the cyp51A genes from 13 reference isolates and 12 clinical isolates of Aspergillus fumigatus. Mutations in this codon confer reduced susceptibility to itraconazole and posaconazole. The assay is simple to perform, and a result of "wild type" or "mutant" is available after approximately 1 h following DNA extraction using commercially available reagents and conventional primers.

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Year:  2010        PMID： 20194699      PMCID： PMC2863670          DOI： 10.1128/AAC.01712-09

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  32 in total

1.  High-resolution melting assay for the detection of gyrA mutations causing quinolone resistance in Salmonella enterica serovars Typhi and Paratyphi.

Authors:  Robert Slinger; Deana Bellfoy; Marc Desjardins; Francis Chan
Journal:  Diagn Microbiol Infect Dis       Date:  2006-12-06       Impact factor: 2.803

2.  Rapid detection and identification of clinically important bacteria by high-resolution melting analysis after broad-range ribosomal RNA real-time PCR.

Authors:  Ju-Chien Cheng; Chien-Ling Huang; Chung-Ching Lin; Chi-Ching Chen; Yi-Chih Chang; Shy-Shin Chang; Ching-Ping Tseng
Journal:  Clin Chem       Date:  2006-09-21       Impact factor: 8.327

3.  Classification of Mycoplasma synoviae strains using single-strand conformation polymorphism and high-resolution melting-curve analysis of the vlhA gene single-copy region.

Authors:  Nathan Jeffery; Robin B Gasser; Penelope A Steer; Amir H Noormohammadi
Journal:  Microbiology (Reading)       Date:  2007-08       Impact factor: 2.777

4.  Rapid differentiation of influenza A virus subtypes and genetic screening for virus variants by high-resolution melting analysis.

Authors:  Jih-Hui Lin; Ching-Ping Tseng; Yen-Ju Chen; Chy-Yung Lin; Shy-Shin Chang; Ho-Sheng Wu; Ju-Chien Cheng
Journal:  J Clin Microbiol       Date:  2008-01-03       Impact factor: 5.948

Review 5.  High resolution melting applications for clinical laboratory medicine.

Authors:  Maria Erali; Karl V Voelkerding; Carl T Wittwer
Journal:  Exp Mol Pathol       Date:  2008-04-13       Impact factor: 3.362

6.  A new Aspergillus fumigatus resistance mechanism conferring in vitro cross-resistance to azole antifungals involves a combination of cyp51A alterations.

Authors:  E Mellado; G Garcia-Effron; L Alcázar-Fuoli; W J G Melchers; P E Verweij; M Cuenca-Estrella; J L Rodríguez-Tudela
Journal:  Antimicrob Agents Chemother       Date:  2007-03-19       Impact factor: 5.191

7.  Rapid detection of triazole antifungal resistance in Aspergillus fumigatus.

Authors:  Guillermo Garcia-Effron; Amanda Dilger; Laura Alcazar-Fuoli; Steven Park; Emilia Mellado; David S Perlin
Journal:  J Clin Microbiol       Date:  2008-01-30       Impact factor: 5.948

8.  High-resolution melting analysis of the spa repeat region of Staphylococcus aureus.

Authors:  Alex J Stephens; John Inman-Bamber; Philip M Giffard; Flavia Huygens
Journal:  Clin Chem       Date:  2008-02       Impact factor: 8.327

9.  Fluorometric assay for testing rifampin susceptibility of Mycobacterium tuberculosis complex.

Authors:  K G P Hoek; N C Gey van Pittius; H Moolman-Smook; K Carelse-Tofa; A Jordaan; G D van der Spuy; E Streicher; T C Victor; P D van Helden; R M Warren
Journal:  J Clin Microbiol       Date:  2008-02-27       Impact factor: 5.948

10.  Optimization of high-resolution melting analysis for low-cost and rapid screening of allelic variants of Bacillus anthracis by multiple-locus variable-number tandem repeat analysis.

Authors:  Daniela Fortini; Andrea Ciammaruconi; Riccardo De Santis; Antonio Fasanella; Antonio Battisti; Raffaele D'Amelio; Florigio Lista; Antonio Cassone; Alessandra Carattoli
Journal:  Clin Chem       Date:  2007-05-24       Impact factor: 8.327
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  5 in total

1.  Rapid detection and identification of Aspergillus from lower respiratory tract specimens by use of a combined probe-high-resolution melting analysis.

Authors:  María Alonso; Pilar Escribano; Jesus Guinea; Sandra Recio; Ainhoa Simon; Teresa Peláez; Emilio Bouza; Darío García de Viedma
Journal:  J Clin Microbiol       Date:  2012-07-25       Impact factor: 5.948

2.  Development and Validation of a High-Resolution Melting Assay To Detect Azole Resistance in Aspergillus fumigatus.

Authors:  L Bernal-Martínez; H Gil; O Rivero-Menéndez; S Gago; M Cuenca-Estrella; E Mellado; A Alastruey-Izquierdo
Journal:  Antimicrob Agents Chemother       Date:  2017-11-22       Impact factor: 5.191

Review 3.  Antifungal drug resistance: do molecular methods provide a way forward?

Authors:  David S Perlin
Journal:  Curr Opin Infect Dis       Date:  2009-12       Impact factor: 4.915

4.  Detection of sdhB Gene Mutations in SDHI-Resistant Isolates of Botrytis cinerea Using High Resolution Melting (HRM) Analysis.

Authors:  Anastasios Samaras; Panagiotis Madesis; George S Karaoglanidis
Journal:  Front Microbiol       Date:  2016-11-15       Impact factor: 5.640

Review 5.  Detecting Azole-Antifungal Resistance in Aspergillus fumigatus by Pyrosequencing.

Authors:  Mireille H van der Torre; Lilyann Novak-Frazer; Riina Rautemaa-Richardson
Journal:  J Fungi (Basel)       Date:  2020-01-10
  5 in total

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