Literature DB >> 17156963

High-resolution melting assay for the detection of gyrA mutations causing quinolone resistance in Salmonella enterica serovars Typhi and Paratyphi.

Robert Slinger1, Deana Bellfoy, Marc Desjardins, Francis Chan.   

Abstract

We developed a novel rapid assay to detect the gyrA mutations that cause quinolone resistance in typhoid and paratyphoid fever Salmonella spp. using high-resolution melting (Idaho Technology, Salt Lake City, UT) analysis of polymerase chain reaction amplicons. The presence of gyrA mutations led to small but consistent changes in amplicon melting temperatures that allowed quinolone-resistant isolates to be differentiated from susceptible ones.

Entities:  

Mesh：

Substances：

Year:  2006        PMID： 17156963     DOI： 10.1016/j.diagmicrobio.2006.09.011

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  7 in total

1.  Use of a high-resolution melt assay to characterize codon 54 of the cyp51A gene of Aspergillus fumigatus on a Rotor-Gene 6000 instrument.

Authors:  M J Tuohy; V Reja; S Park; D S Perlin; M Wnek; G W Procop; B Yen-Lieberman
Journal:  Antimicrob Agents Chemother       Date:  2010-03-01       Impact factor: 5.191

2.  Rapid Detection of Common HIV-1 Drug Resistance Mutations by Use of High-Resolution Melting Analysis and Unlabeled Probes.

Authors:  David Sacks; Johanna Ledwaba; Lynn Morris; Gillian M Hunt
Journal:  J Clin Microbiol       Date:  2016-12-28       Impact factor: 5.948

3.  Development and Validation of a High-Resolution Melting Assay To Detect Azole Resistance in Aspergillus fumigatus.

Authors:  L Bernal-Martínez; H Gil; O Rivero-Menéndez; S Gago; M Cuenca-Estrella; E Mellado; A Alastruey-Izquierdo
Journal:  Antimicrob Agents Chemother       Date:  2017-11-22       Impact factor: 5.191

4.  Simple, rapid, and inexpensive detection of Neisseria gonorrhoeae resistance mechanisms using heat-denatured isolates and SYBR green-based real-time PCR.

Authors:  Gayle Kugelman; John W Tapsall; Namraj Goire; Melanie W Syrmis; Athena Limnios; Stephen B Lambert; Michael D Nissen; Theo P Sloots; David M Whiley
Journal:  Antimicrob Agents Chemother       Date:  2009-06-15       Impact factor: 5.191

5.  A multiplex single nucleotide polymorphism typing assay for detecting mutations that result in decreased fluoroquinolone susceptibility in Salmonella enterica serovars Typhi and Paratyphi A.

Authors:  Yajun Song; Philippe Roumagnac; François-Xavier Weill; John Wain; Christiane Dolecek; Camila J Mazzoni; Kathryn E Holt; Mark Achtman
Journal:  J Antimicrob Chemother       Date:  2010-05-28       Impact factor: 5.790

6.  High resolution melting analysis for rapid mutation screening in gyrase and Topoisomerase IV genes in quinolone-resistant Salmonella enterica.

Authors:  Soo Tein Ngoi; Kwai Lin Thong
Journal:  Biomed Res Int       Date:  2014-10-12       Impact factor: 3.411

7.  Mixture models for analysis of melting temperature data.

Authors:  Christoffer Nellåker; Fredrik Uhrzander; Joanna Tyrcha; Håkan Karlsson
Journal:  BMC Bioinformatics       Date:  2008-09-11       Impact factor: 3.169
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.