Literature DB >> 19765264

Live cell multicolor imaging of lipid droplets with a new dye, LD540.

Johanna Spandl1, Daniel J White, Jan Peychl, Christoph Thiele.   

Abstract

A lipophilic dye based on the Bodipy fluorophore, LD540, was developed for microscopic imaging of lipid droplets. In contrast to previous lipid droplet dyes, it can spectrally be resolved from both green and red fluorophores allowing multicolor imaging in both fixed and living cells. Its improved specificity, brightness and photostability support live cell imaging, which was used to demonstrate by two-color imaging lipid droplet motility along microtubules.

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Year:  2009        PMID: 19765264     DOI: 10.1111/j.1600-0854.2009.00980.x

Source DB:  PubMed          Journal:  Traffic        ISSN: 1398-9219            Impact factor:   6.215


  81 in total

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Journal:  J Mater Sci Mater Med       Date:  2013-11-08       Impact factor: 3.896

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Authors:  Songyu Wang; Guillaume Thibault; Davis T W Ng
Journal:  J Biol Chem       Date:  2011-06-27       Impact factor: 5.157

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Authors:  Erdinc Sezgin; Petra Schwille
Journal:  Cold Spring Harb Perspect Biol       Date:  2011-11-01       Impact factor: 10.005

6.  A pitfall in using BODIPY dyes to label lipid droplets for fluorescence microscopy.

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7.  Dictyostelium discoideum Dgat2 can substitute for the essential function of Dgat1 in triglyceride production but not in ether lipid synthesis.

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Journal:  Eukaryot Cell       Date:  2014-02-21

8.  Lipid droplet quantification based on iterative image processing.

Authors:  Tarik Exner; Carlo A Beretta; Qi Gao; Cassian Afting; Inés Romero-Brey; Ralf Bartenschlager; Leonard Fehring; Margarete Poppelreuther; Joachim Füllekrug
Journal:  J Lipid Res       Date:  2019-03-29       Impact factor: 5.922

9.  A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins.

Authors:  Anne Gaebler; Anke Penno; Lars Kuerschner; Christoph Thiele
Journal:  J Lipid Res       Date:  2016-08-26       Impact factor: 5.922

10.  Body size-dependent energy storage causes Kleiber's law scaling of the metabolic rate in planarians.

Authors:  Albert Thommen; Steffen Werner; Olga Frank; Jenny Philipp; Oskar Knittelfelder; Yihui Quek; Karim Fahmy; Andrej Shevchenko; Benjamin M Friedrich; Frank Jülicher; Jochen C Rink
Journal:  Elife       Date:  2019-01-04       Impact factor: 8.140

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