| Literature DB >> 20171882 |
Yishan Su1, Xia Li, Qingzhi Liu, Zhongwen Hou, Xiqiang Zhu, Xueping Guo, Peixue Ling.
Abstract
In order to alleviate oxygen limitation and improve the yield of poly-gamma-glutamic acid (gamma-PGA) during fermentation, the Vitreoscilla hemoglobin gene (vgb) was integrated into the chromosome of Bacillus subtilis and expressed during gamma-PGA production. The activity of the expressed Vitreoscilla hemoglobin (VHb) was confirmed by CO-difference spectrum. Expression of VHb enhanced cell growth under high viscosity fermentation conditions 1.26-fold and increased the yield of gamma-PGA 2.07-fold. These results indicate that the expression of VHb could be advantageous in high viscosity fermentation media. (c) 2010 Elsevier Ltd. All rights reserved.Entities:
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Year: 2010 PMID： 20171882 DOI： 10.1016/j.biortech.2010.01.128
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642