OBJECTIVE: To determine if mesenchymal stem cells (MSC) derived from human fetal pancreatic tissue (pMSC) would engraft and differentiate in sheep pancreas following transplantation in utero. MATERIALS AND METHODS: A three-step culture system was established for generating human fetal pMSC. Sheep fetuses were transplanted during the fetal transplant receptivity period with human pMSC and evaluated for in situ and functional engraftment in their pancreas, liver, and bone marrow. RESULTS: Isolation and expansion of adherent cells from the human fetal pancreas yielded a cell population with morphologic and phenotypic characteristics similar to MSC derived from bone marrow. This putative stem cell population could undergo multilineage differentiation in vitro. Three to 27 months after fetal transplantation, the pancreatic engraftment frequency (chimeric index) was 79%, while functional engraftment was noted in 50% of transplanted sheep. Hepatic and marrow engraftment and expression was noted as well. CONCLUSION: We have established a procedure for isolation of human fetal pMSC that display characteristics similar to bone marrow-derived MSC. In vivo results suggest the pMSC engraft, differentiate, and secrete human insulin from the sheep pancreas.
OBJECTIVE: To determine if mesenchymal stem cells (MSC) derived from human fetal pancreatic tissue (pMSC) would engraft and differentiate in sheep pancreas following transplantation in utero. MATERIALS AND METHODS: A three-step culture system was established for generating human fetal pMSC. Sheep fetuses were transplanted during the fetal transplant receptivity period with human pMSC and evaluated for in situ and functional engraftment in their pancreas, liver, and bone marrow. RESULTS: Isolation and expansion of adherent cells from the human fetal pancreas yielded a cell population with morphologic and phenotypic characteristics similar to MSC derived from bone marrow. This putative stem cell population could undergo multilineage differentiation in vitro. Three to 27 months after fetal transplantation, the pancreatic engraftment frequency (chimeric index) was 79%, while functional engraftment was noted in 50% of transplanted sheep. Hepatic and marrow engraftment and expression was noted as well. CONCLUSION: We have established a procedure for isolation of human fetal pMSC that display characteristics similar to bone marrow-derived MSC. In vivo results suggest the pMSC engraft, differentiate, and secrete humaninsulin from the sheep pancreas.
Authors: Manuel Alvarez-Dolado; Ricardo Pardal; Jose M Garcia-Verdugo; John R Fike; Hyun O Lee; Klaus Pfeffer; Carlos Lois; Sean J Morrison; Arturo Alvarez-Buylla Journal: Nature Date: 2003-10-12 Impact factor: 49.962
Authors: C Wiese; A Rolletschek; G Kania; P Blyszczuk; K V Tarasov; Y Tarasova; R P Wersto; K R Boheler; A M Wobus Journal: Cell Mol Life Sci Date: 2004-10 Impact factor: 9.261
Authors: Behrous Davani; Laertis Ikonomou; Bruce M Raaka; Elizabeth Geras-Raaka; Russell A Morton; Bernice Marcus-Samuels; Marvin C Gershengorn Journal: Stem Cells Date: 2007-09-27 Impact factor: 6.277
Authors: Volkert A L Huurman; Robert Hilbrands; Gabriëlle G M Pinkse; Pieter Gillard; Gaby Duinkerken; Pieter van de Linde; Petronella M W van der Meer-Prins; Minke F J Versteeg-van der Voort Maarschalk; Koen Verbeeck; Behrooz Z Alizadeh; Chantal Mathieu; Frans K Gorus; Dave L Roelen; Frans H J Claas; Bart Keymeulen; Daniel G Pipeleers; Bart O Roep Journal: PLoS One Date: 2008-06-18 Impact factor: 3.240
Authors: Jennifer M Ryan; Allison R Pettit; Pascale V Guillot; Jerry K Y Chan; Nicholas M Fisk Journal: Stem Cell Rev Rep Date: 2013-08 Impact factor: 5.739
Authors: Rafael Moreno; Itziar Martínez-González; Marta Rosal; Marga Nadal; Jordi Petriz; Eduard Gratacós; Josep M Aran Journal: Stem Cells Dev Date: 2011-06-01 Impact factor: 3.272