Literature DB >> 20165908

Characterization of water-soluble complexes of polyacrylic acid with alpha-Amylase from Aspergillus oryzae.

Melda Altikatoglu1, Ozlem Tavukcuoglu, Mamed Mustafaev.   

Abstract

Polycomplex formation of alpha-Amylase from Aspergillus oryzae (TAKA) with polyacrylic acid (PAA) was studied by pH titration, fluorescence, and high performance liquid chromatography (HPLC) methods in water solutions. According to the our results, the complex formation and its solubility were depended on nature of enzyme and the pH of solutions. Both of them correlates isoelectric points (PI). The stability of PAA-amylase complexes was negligibly weak at pH 7 [pH > pI (isoelectric pH)]. Stable water-soluble polycomplexes were formed at pH 5 (pI approximately 4.5) and coexisted with free protein molecules. Insoluble complexes has been observed at pH < 4.5. The frozen storage stabilities of the obtained complexes were also studied by measuring the activities at different pH.

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Year:  2010        PMID： 20165908     DOI： 10.1007/s10930-010-9231-y

Source DB:  PubMed          Journal:  Protein J        ISSN： 1572-3887            Impact factor:   2.371


  9 in total

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Journal:  Structure       Date:  2003-08       Impact factor: 5.006

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Journal:  Biochemistry       Date:  1998-08-25       Impact factor: 3.162

3.  Toward understanding tryptophan fluorescence in proteins.

Authors:  Y Chen; M D Barkley
Journal:  Biochemistry       Date:  1998-07-14       Impact factor: 3.162

4.  Structural analysis of a chimeric bacterial alpha-amylase. High-resolution analysis of native and ligand complexes.

Authors:  A M Brzozowski; D M Lawson; J P Turkenburg; H Bisgaard-Frantzen; A Svendsen; T V Borchert; Z Dauter; K S Wilson; G J Davies
Journal:  Biochemistry       Date:  2000-08-08       Impact factor: 3.162

5.  Structure of the Aspergillus oryzae alpha-amylase complexed with the inhibitor acarbose at 2.0 A resolution.

Authors:  A M Brzozowski; G J Davies
Journal:  Biochemistry       Date:  1997-09-09       Impact factor: 3.162

6.  Carbohydrate and protein-based inhibitors of porcine pancreatic alpha-amylase: structure analysis and comparison of their binding characteristics.

Authors:  M Machius; L Vértesy; R Huber; G Wiegand
Journal:  J Mol Biol       Date:  1996-07-19       Impact factor: 5.469

7.  [Complex formation of copolymers of 4-vinylpyridine and 4-vinyl-cetylpyridinium bromide with serum albumin].

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Journal:  Dokl Akad Nauk SSSR       Date:  1975-11-21

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Journal:  Int J Biol Macromol       Date:  2007-03-25       Impact factor: 6.953

9.  Interpolymer complexes of poly(acrylic acid) and chitosan: influence of the ionic hydrogel-forming medium.

Authors:  Paloma M de la Torre; Susana Torrado; Santiago Torrado
Journal:  Biomaterials       Date:  2003-04       Impact factor: 12.479
  9 in total
  1 in total

1.  Investigation of ternary complex formations of polyacrylic acid with bovine serum albumin in the presence of metal ions by fluorescence and dynamic light scattering measurements.

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Journal:  Protein J       Date:  2010-07       Impact factor: 2.371
  1 in total

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