Literature DB >> 20158249

Fluorescent structural DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates.

Jon P Anderson1, Bambi L Reynolds, Kristin Baum, John G Williams.   

Abstract

Highly labeled DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates (dNTPs) were developed as a source of dNTPs for DNA polymerase. The particles were prepared by strand-displacement polymerization from a self-complementary circular template. Imaged by atomic force microscopy, these functionalized particles appear as condensed fuzzy balls with diameters between 50 and 150 nm. They emit a bright fluorescent signal, detected in 2 ms exposures with a signal-to-noise ratio of 25 when imaged using a TIR fluorescence microscope.

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Year:  2010        PMID： 20158249      PMCID： PMC2843431          DOI： 10.1021/nl9039718

Source DB:  PubMed          Journal:  Nano Lett        ISSN： 1530-6984            Impact factor:   11.189


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