INTRODUCTION: Forsythia suspensa is a commonly used traditional Chinese medicine including phenylethanoid glycosides, lignans, flavonoids, terpenes and volatile oils. Quantification of multi-components is important for the quality control of Forsythia suspensa. OBJECTIVE: To establish a liquid chromatography-electrospray ionisation-mass spectrometry method for simultaneous quantification of 14 bioactive constituents of Forsythia suspensa in different places of China and different parts of this herb. METHODOLOGY: The optimal chromatographic conditions were achieved on a Kromasil C(18) column (150 yen 4.6 mm, 5 microm) with gradient elution of methanol, acetonitrile and 0.1% formic acid in 27 min. Detection was performed in negative ionisation mode by monitoring the precursor-product combination in multiple reaction monitoring (MRM) mode. The validation of the method included tests of linearity, sensitivity, precision, repeatability, stability and accuracy. RESULTS: All calibration curves showed good linear regression (r > 0.9990) within test ranges. The established method showed good precision and accuracy with overall intra-day and inter-day variations of 0.7-4.3 and 1.1-3.9% respectively, and overall recoveries of 96.65-101.2% for the compounds analysed. CONCLUSION: The proposed method was successfully applied for the quantitative analysis of 14 constituents in 12 Forsythia suspensa samples. (c) 2010 John Wiley & Sons, Ltd.
INTRODUCTION:Forsythia suspensa is a commonly used traditional Chinese medicine including phenylethanoid glycosides, lignans, flavonoids, terpenes and volatile oils. Quantification of multi-components is important for the quality control of Forsythia suspensa. OBJECTIVE: To establish a liquid chromatography-electrospray ionisation-mass spectrometry method for simultaneous quantification of 14 bioactive constituents of Forsythia suspensa in different places of China and different parts of this herb. METHODOLOGY: The optimal chromatographic conditions were achieved on a Kromasil C(18) column (150 yen 4.6 mm, 5 microm) with gradient elution of methanol, acetonitrile and 0.1% formic acid in 27 min. Detection was performed in negative ionisation mode by monitoring the precursor-product combination in multiple reaction monitoring (MRM) mode. The validation of the method included tests of linearity, sensitivity, precision, repeatability, stability and accuracy. RESULTS: All calibration curves showed good linear regression (r > 0.9990) within test ranges. The established method showed good precision and accuracy with overall intra-day and inter-day variations of 0.7-4.3 and 1.1-3.9% respectively, and overall recoveries of 96.65-101.2% for the compounds analysed. CONCLUSION: The proposed method was successfully applied for the quantitative analysis of 14 constituents in 12 Forsythia suspensa samples. (c) 2010 John Wiley & Sons, Ltd.
Authors: Barbara Michalak; Agnieszka Filipek; Piotr Chomicki; Małgorzata Pyza; Marta Woźniak; Barbara Żyżyńska-Granica; Jakub P Piwowarski; Agnieszka Kicel; Monika A Olszewska; Anna K Kiss Journal: Front Pharmacol Date: 2018-04-24 Impact factor: 5.810