| Literature DB >> 20149523 |
Takamichi Ishii1, Kentaro Yasuchika, Hirofumi Suemori, Norio Nakatsuji, Iwao Ikai, Shinji Uemoto.
Abstract
We aimed to demonstrate that alpha-fetoprotein (AFP)-producing cells in cholangiocarcinomas possessed cancer stem cell (CSC)-like properties. AFP enhancer/promoter-driven EGFP gene was transfected into human cholangiocarcinoma cell lines. One cell line, RBE, expressed both AFP and EGFP. Clonal analyses revealed that one EGFP-positive cell generated both EGFP-positive and EGFP-negative cell fractions. However, one EGFP-negative cell never produced EGFP-positive cells. The EGFP-positive cells had a greater tumorigenic potential. Only the EGFP-positive cells expressed Notch1. AFP and Notch1 expression was observed in clinical intrahepatic cholangiocarcinomas. The AFP-producing cells were suggested to be CSCs. The Notch pathway might play an important role in maintaining the CSC characteristics. Copyright (c) 2010 Elsevier Ireland Ltd. All rights reserved.Entities:
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Year: 2010 PMID： 20149523 DOI： 10.1016/j.canlet.2010.01.019
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679