Literature DB >> 20146939

Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRT-PCR).

Evan M Kroh1, Rachael K Parkin, Patrick S Mitchell, Muneesh Tewari.   

Abstract

MicroRNAs (miRNAs) are small (approximately 22 nt) RNAs that play important roles in gene regulatory networks by binding to and repressing the activity of specific target mRNAs. Recent studies have indicated that miRNAs circulate in a stable, cell-free form in the bloodstream and that the abundance of specific miRNAs in plasma or serum can serve as biomarkers of cancer and other diseases. Measurement of circulating miRNAs as biomarkers is associated with some special challenges, including those related to pre-analytic variation and data normalization. We describe here our procedure for qRT-PCR analysis of circulating miRNAs as biomarkers, and discuss relevant issues of sample preparation, experimental design and data analysis. Copyright 2010 Elsevier Inc. All rights reserved.

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Year:  2010        PMID： 20146939      PMCID： PMC4186708          DOI： 10.1016/j.ymeth.2010.01.032

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  12 in total

1.  Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAs.

Authors:  Christopher K Raymond; Brian S Roberts; Phillip Garrett-Engele; Lee P Lim; Jason M Johnson
Journal:  RNA       Date:  2005-11       Impact factor: 4.942

Review 2.  Real-time PCR quantification of precursor and mature microRNA.

Authors:  Thomas D Schmittgen; Eun Joo Lee; Jinmai Jiang; Anasuya Sarkar; Liuqing Yang; Terry S Elton; Caifu Chen
Journal:  Methods       Date:  2008-01       Impact factor: 3.608

Review 3.  Origins and Mechanisms of miRNAs and siRNAs.

Authors:  Richard W Carthew; Erik J Sontheimer
Journal:  Cell       Date:  2009-02-20       Impact factor: 41.582

4.  Circulating microRNAs as stable blood-based markers for cancer detection.

Authors:  Patrick S Mitchell; Rachael K Parkin; Evan M Kroh; Brian R Fritz; Stacia K Wyman; Era L Pogosova-Agadjanyan; Amelia Peterson; Jennifer Noteboom; Kathy C O'Briant; April Allen; Daniel W Lin; Nicole Urban; Charles W Drescher; Beatrice S Knudsen; Derek L Stirewalt; Robert Gentleman; Robert L Vessella; Peter S Nelson; Daniel B Martin; Muneesh Tewari
Journal:  Proc Natl Acad Sci U S A       Date:  2008-07-28       Impact factor: 11.205

5.  MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer.

Authors:  Douglas D Taylor; Cicek Gercel-Taylor
Journal:  Gynecol Oncol       Date:  2008-07       Impact factor: 5.482

6.  Detection and characterization of placental microRNAs in maternal plasma.

Authors:  Stephen S C Chim; Tristan K F Shing; Emily C W Hung; Tak-Yeung Leung; Tze-Kin Lau; Rossa W K Chiu; Y M Dennis Lo
Journal:  Clin Chem       Date:  2008-01-24       Impact factor: 8.327

7.  Detection of elevated levels of tumour-associated microRNAs in serum of patients with diffuse large B-cell lymphoma.

Authors:  Charles H Lawrie; Shira Gal; Heather M Dunlop; Beena Pushkaran; Amanda P Liggins; Karen Pulford; Alison H Banham; Francesco Pezzella; Jacqueline Boultwood; James S Wainscoat; Christian S R Hatton; Adrian L Harris
Journal:  Br J Haematol       Date:  2008-03-03       Impact factor: 6.998

8.  Real-time quantification of microRNAs by stem-loop RT-PCR.

Authors:  Caifu Chen; Dana A Ridzon; Adam J Broomer; Zhaohui Zhou; Danny H Lee; Julie T Nguyen; Maura Barbisin; Nan Lan Xu; Vikram R Mahuvakar; Mark R Andersen; Kai Qin Lao; Kenneth J Livak; Karl J Guegler
Journal:  Nucleic Acids Res       Date:  2005-11-27       Impact factor: 16.971

9.  Detection of microRNA expression in human peripheral blood microvesicles.

Authors:  Melissa Piper Hunter; Noura Ismail; Xiaoli Zhang; Baltazar D Aguda; Eun Joo Lee; Lianbo Yu; Tao Xiao; Jeffrey Schafer; Mei-Ling Ting Lee; Thomas D Schmittgen; S Patrick Nana-Sinkam; David Jarjoura; Clay B Marsh
Journal:  PLoS One       Date:  2008-11-11       Impact factor: 3.240

10.  Serum microRNAs are promising novel biomarkers.

Authors:  Shlomit Gilad; Eti Meiri; Yariv Yogev; Sima Benjamin; Danit Lebanony; Noga Yerushalmi; Hila Benjamin; Michal Kushnir; Hila Cholakh; Nir Melamed; Zvi Bentwich; Moshe Hod; Yaron Goren; Ayelet Chajut
Journal:  PLoS One       Date:  2008-09-05       Impact factor: 3.240
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  470 in total

1.  Identification of suitable reference genes for qPCR analysis of serum microRNA in gastric cancer patients.

Authors:  Jianning Song; Zhigang Bai; Wei Han; Jun Zhang; Hua Meng; Jintao Bi; Xuemei Ma; Shiwei Han; Zhongtao Zhang
Journal:  Dig Dis Sci       Date:  2011-12-25       Impact factor: 3.199

Review 2.  Circulating microRNAs: novel biomarkers for cardiovascular diseases.

Authors:  Jiahong Xu; Jiangmin Zhao; Graham Evan; Chunyang Xiao; Yan Cheng; Junjie Xiao
Journal:  J Mol Med (Berl)       Date:  2011-12-08       Impact factor: 4.599

Review 3.  MicroRNAs in liver disease.

Authors:  Xin Wei Wang; Niels H H Heegaard; Henrik Orum
Journal:  Gastroenterology       Date:  2012-04-11       Impact factor: 22.682

4.  Characterization of grapevine microR164 and its target genes.

Authors:  Xin Sun; Nicholas Kibet Korir; Jian Han; Ling-Fei Shangguan; Emrul Kayesh; Xiang-Peng Leng; Jing-Gui Fang
Journal:  Mol Biol Rep       Date:  2012-06-24       Impact factor: 2.316

Review 5.  Epigenetic biomarkers in skin cancer.

Authors:  Edward S Greenberg; Kelly K Chong; Kelly T Huynh; Ryo Tanaka; Dave S B Hoon
Journal:  Cancer Lett       Date:  2012-01-27       Impact factor: 8.679

6.  The diagnostic value of microRNA-4787-5p and microRNA-4306 in patients with acute aortic dissection.

Authors:  Lei Wang; Shijie Zhang; Zhigao Xu; Jingjing Zhang; Li Li; Guoqiang Zhao
Journal:  Am J Transl Res       Date:  2017-11-15       Impact factor: 4.060

Review 7.  Emergence of Circulating MicroRNAs in Breast Cancer as Diagnostic and Therapeutic Efficacy Biomarkers.

Authors:  Vaishali Aggarwal; Kumari Priyanka; Hardeep Singh Tuli
Journal:  Mol Diagn Ther       Date:  2020-04       Impact factor: 4.074

8.  Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR.

Authors:  Takuma Iguchi; Noriyo Niino; Satoshi Tamai; Ken Sakurai; Kazuhiko Mori
Journal:  J Vis Exp       Date:  2018-02-12       Impact factor: 1.355

Review 9.  MicroRNAs in colorectal cancer: role in metastasis and clinical perspectives.

Authors:  Shan Muhammad; Kavanjit Kaur; Rui Huang; Qian Zhang; Paviter Kaur; Hamza Obaid Yazdani; Muhammad Umar Bilal; Jiang Zheng; Liu Zheng; Xi-Shan Wang
Journal:  World J Gastroenterol       Date:  2014-12-07       Impact factor: 5.742

10.  Comparative quantitative analysis of gene expression profiles of glycoside hydrolase family 10 xylanases in the sheep rumen during a feeding cycle.

Authors:  Zhongyuan Li; Heng Zhao; Peilong Yang; Junqi Zhao; Huoqing Huang; Xianli Xue; Xinshang Zhang; Qiyu Diao; Bin Yao
Journal:  Appl Environ Microbiol       Date:  2012-12-07       Impact factor: 4.792
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