| Literature DB >> 20144491 |
Sonia Malik1, Nandini Sharma, Upendra K Sharma, Narendra P Singh, Shashi Bhushan, Madhu Sharma, Arun K Sinha, Paramvir S Ahuja.
Abstract
This paper presents quantification of five anthraquinone derivatives (emodin glycoside, chrysophanol glycoside, emodin, chrysophanol and physcion) in rhizomes of hardened micro-propagated Rheum emodi plants using high-performance liquid chromatography (HPLC). Aseptic shoot cultures were raised using rhizome buds. Shoot multiplication occurred in both agar gelled and liquid Murashige and Skoog (MS) medium supplemented with 10.0 microM 6-benzylaminopurine (BAP) and 5.0 microM indole-3-butyric acid (IBA). Rooted plantlets obtained on plant growth regulator (PGR)-free medium were transferred to soil with 92% survival. HPLC analysis revealed the presence of five anthraquinone derivatives: emodin glycoside, chrysophanol glycoside, emodin, chrysophanol and physcion in rhizomes of tissue culture-raised plants. Only emodin glycoside (1) and chrysophanol glycoside (2) were present in 6-month-old hardened tissue cultured plants. In addition, the other three derivatives (emodin (3), chrysophanol (4) and physcion (5)) were also detected after 9 months. Copyright 2010 Elsevier GmbH. All rights reserved.Entities:
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Year: 2010 PMID： 20144491 DOI： 10.1016/j.jplph.2009.12.007
Source DB: PubMed Journal: J Plant Physiol ISSN： 0176-1617 Impact factor: 3.549