BACKGROUND: The study sets out to either confirm or refute a recent study's findings that chicken meat is an unlikely source of Salmonella enterica subspecies enterica serovar Infantis (Salmonella Infantis) in humans in the Kyushu-Okinawa region, Japan. METHODS: A total of 74 Salmonella Infantis isolates (30 from human and 44 from other sources), mainly from the Kyushu-Okinawa region in south-western Japan, were analyzed using a molecular-epidemiological approach combining two fingerprinting methods, namely pulsed-field gel electrophoresis and amplified fragment length polymorphism (AFLP), a novel polymerase chain reaction-based technique. RESULTS: The resulting pulsed-field profiles showed that 17 of 30 human isolates were similar to those found in chicken meat, whereas there were no common pulsed-field profiles between human and chicken egg isolates. Overall, 3 of 18 AFLP profiles included 7 human isolates and 14 chicken egg isolates. In addition, the combined results of the pulsed-field gel electrophoresis and AFLP analyses showed that 8 human Salmonella Infantis and 13 chicken meat isolates belonged to the same clonal lines. CONCLUSIONS: These results suggest that chicken meat is an infection source of Salmonella Infantis for humans in the Kyushu-Okinawa region, Japan. The results also showed the relatively high suitability of AFLP for application to epidemiological studies of Salmonella Infantis.
BACKGROUND: The study sets out to either confirm or refute a recent study's findings that chicken meat is an unlikely source of Salmonella enterica subspecies enterica serovar Infantis (Salmonella Infantis) in humans in the Kyushu-Okinawa region, Japan. METHODS: A total of 74 Salmonella Infantis isolates (30 from human and 44 from other sources), mainly from the Kyushu-Okinawa region in south-western Japan, were analyzed using a molecular-epidemiological approach combining two fingerprinting methods, namely pulsed-field gel electrophoresis and amplified fragment length polymorphism (AFLP), a novel polymerase chain reaction-based technique. RESULTS: The resulting pulsed-field profiles showed that 17 of 30 human isolates were similar to those found in chicken meat, whereas there were no common pulsed-field profiles between human and chicken egg isolates. Overall, 3 of 18 AFLP profiles included 7 human isolates and 14 chicken egg isolates. In addition, the combined results of the pulsed-field gel electrophoresis and AFLP analyses showed that 8 humanSalmonella Infantis and 13 chicken meat isolates belonged to the same clonal lines. CONCLUSIONS: These results suggest that chicken meat is an infection source of Salmonella Infantis for humans in the Kyushu-Okinawa region, Japan. The results also showed the relatively high suitability of AFLP for application to epidemiological studies of Salmonella Infantis.
Authors: S Besharati; A Sadeghi; F Ahmadi; E Tajeddin; R Mohammad Salehi; F Fani; Gh Pouladfar; B Nikmanesh; A Majidpour; S Soleymanzadeh Moghadam; S Mirab Samiee; M Rahnamaye Farzami; M Rahbar; P Eslami; N Rakhshani; B Eshrati; M M Gouya; F Fallah; A Karimi; P Owlia; M Alebouyeh Journal: Iran J Vet Res Date: 2020 Impact factor: 1.376