Literature DB >> 20140385

Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples.

Luciana I Gomes1, Letícia H S Marques, Martin J Enk, Paulo Marcos Z Coelho, Ana Rabello.   

Abstract

A previously reported sensitive PCR assay for the detection of Schistosoma mansoni DNA was updated and evaluated. Changes in the DNA extraction method, including the use of a worldwide available commercial kit and the inclusion of additional quality control measures, increased the robustness of the test, as confirmed by the analysis of 67 faecal samples from an endemic area in Brazil. The PCR assay is at hand as a proven, reliable diagnostic test for the control of schistosomiasis in specific settings.

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Year:  2009        PMID： 20140385     DOI： 10.1590/s0074-02762009000800021

Source DB:  PubMed          Journal:  Mem Inst Oswaldo Cruz        ISSN： 0074-0276            Impact factor:   2.743


  15 in total

1.  Comparative evaluation of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium alkaline phosphatase antigenicity by the alkaline phosphatase immunoassay (APIA).

Authors:  I M Cesari; D E Ballén; L Mendoza; A Ferrer; J-P Pointier; M Kombila; D Richard-Lenoble; A Théron
Journal:  Parasitol Res       Date:  2014-01-23       Impact factor: 2.289

Review 2.  Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.

Authors:  Jaco J Verweij; C Rune Stensvold
Journal:  Clin Microbiol Rev       Date:  2014-04       Impact factor: 26.132

3.  Field evaluation of a PCR test for Schistosoma japonicum egg detection in low-prevalence regions of China.

Authors:  Mai S Fung; Ning Xiao; Shuo Wang; Elizabeth J Carlton
Journal:  Am J Trop Med Hyg       Date:  2012-10-29       Impact factor: 2.345

4.  Evaluation of three extraction methods for molecular detection of Schistosoma mansoni infection in human urine and serum samples.

Authors:  Rania M Sarhan; Hanan H Kamel; Ghada A Saad; Ossama A Ahmed
Journal:  J Parasit Dis       Date:  2013-11-20

5.  Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area.

Authors:  Martin Johannes Enk; Guilherme Oliveira e Silva; Nilton Barnabé Rodrigues
Journal:  PLoS One       Date:  2012-06-11       Impact factor: 3.240

6.  Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area.

Authors:  Liliane Maria Vidal Siqueira; Luciana Inácia Gomes; Edward Oliveira; Eduardo Ribeiro de Oliveira; Áureo Almeida de Oliveira; Martin Johannes Enk; Nídia Figueiredo Carneiro; Ana Rabello; Paulo Marcos Zech Coelho
Journal:  Mem Inst Oswaldo Cruz       Date:  2015-04       Impact factor: 2.743

Review 7.  Hepatic and intestinal schistosomiasis: review.

Authors:  Tamer Elbaz; Gamal Esmat
Journal:  J Adv Res       Date:  2013-01-11       Impact factor: 10.479

8.  Evaluation of real-time PCR assay to detect Schistosoma mansoni infections in a low endemic setting.

Authors:  Maria Cristina Carvalho Espírito-Santo; Mónica Viviana Alvarado-Mora; Emmanuel Dias-Neto; Lívia Souza Botelho-Lima; João Paulo Moreira; Maria Amorim; Pedro Luiz Silva Pinto; Ashley R Heath; Vera Lúcia Pagliusi Castilho; Elenice Messias do Nascimento Gonçalves; Expedito José de Albuquerque Luna; Flair José Carrilho; João Renato Rebello Pinho; Ronaldo Cesar Borges Gryschek
Journal:  BMC Infect Dis       Date:  2014-10-23       Impact factor: 3.090

9.  Association between response to albendazole treatment and β-tubulin genotype frequencies in soil-transmitted helminths.

Authors:  Aïssatou Diawara; Carli M Halpenny; Thomas S Churcher; Charles Mwandawiro; Jimmy Kihara; Ray M Kaplan; Thomas G Streit; Youssef Idaghdour; Marilyn E Scott; Maria-Gloria Basáñez; Roger K Prichard
Journal:  PLoS Negl Trop Dis       Date:  2013-05-30

10.  Is PCR the Next Reference Standard for the Diagnosis of Schistosoma in Stool? A Comparison with Microscopy in Senegal and Kenya.

Authors:  Lynn Meurs; Eric Brienen; Moustapha Mbow; Elizabeth A Ochola; Souleymane Mboup; Diana M S Karanja; W Evan Secor; Katja Polman; Lisette van Lieshout
Journal:  PLoS Negl Trop Dis       Date:  2015-07-28
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