I M S Eldeen1, F R Van Heerden, J Van Staden. 1. Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Acacia nilotica subsp. kraussiana was reported in African traditional medicine for the treatment of various ailments. Isolation of an active compound in this study from the bark extract may lead to the validation of its efficiency as a traditional crude drug. AIMS OF THE STUDY: This study aimed to isolate active compound(s) from an ethyl acetate bark extract of Acacia nilotica subsp. kraussiana and to investigate some of its biological activity. MATERIALS AND METHODS: The isolation process was carried out using bioassay-guided fractionation. The isolated compound was tested for antibacterial activity using the micro-dilution assay; anti-inflammatory activity using the COX-1 and COX-2 assays and investigated for inhibitory effect against acetylcholinesterase using the microplate assay. RESULTS: A new bioactive compound was isolated and identified as a cassane diterpene, niloticane. Niloticane showed antibacterial activity against Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus with MIC values of 4 and 8microg/mL, respectively. With Gram-negative bacteria, niloticane showed weak activity. MIC values obtained were 16 and 33microg/mL against Klebsiella pneumonia and Escherichia coli, respectively. In the cyclooxygenase test, niloticane possessed activity with IC50 values of 28 and 210microM against COX-1 and COX-2, respectively. IC50 values observed with indomethacin (positive control) were 3.6microM for COX-1 and 189microM for COX-2. In the acetylcholinesterase test, niloticane showed anti-cholinesterase activity with an IC50 value of 4microM. IC50 values obtained by the galanthamine (positive control) was 2.0microM. CONCLUSION: The results obtained support the traditional uses of the bark of Acacia nilotica subsp. kraussiana in African traditional medicine for the treatment of some ailments that relate to microbial diseases, inflammation and central nervous system disorders. Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
ETHNOPHARMACOLOGICAL RELEVANCE: Acacia nilotica subsp. kraussiana was reported in African traditional medicine for the treatment of various ailments. Isolation of an active compound in this study from the bark extract may lead to the validation of its efficiency as a traditional crude drug. AIMS OF THE STUDY: This study aimed to isolate active compound(s) from an ethyl acetate bark extract of Acacia nilotica subsp. kraussiana and to investigate some of its biological activity. MATERIALS AND METHODS: The isolation process was carried out using bioassay-guided fractionation. The isolated compound was tested for antibacterial activity using the micro-dilution assay; anti-inflammatory activity using the COX-1 and COX-2 assays and investigated for inhibitory effect against acetylcholinesterase using the microplate assay. RESULTS: A new bioactive compound was isolated and identified as a cassanediterpene, niloticane. Niloticane showed antibacterial activity against Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus with MIC values of 4 and 8microg/mL, respectively. With Gram-negative bacteria, niloticane showed weak activity. MIC values obtained were 16 and 33microg/mL against Klebsiella pneumonia and Escherichia coli, respectively. In the cyclooxygenase test, niloticane possessed activity with IC50 values of 28 and 210microM against COX-1 and COX-2, respectively. IC50 values observed with indomethacin (positive control) were 3.6microM for COX-1 and 189microM for COX-2. In the acetylcholinesterase test, niloticane showed anti-cholinesterase activity with an IC50 value of 4microM. IC50 values obtained by the galanthamine (positive control) was 2.0microM. CONCLUSION: The results obtained support the traditional uses of the bark of Acacia nilotica subsp. kraussiana in African traditional medicine for the treatment of some ailments that relate to microbial diseases, inflammation and central nervous system disorders. Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
Authors: Ana Paula Murray; María Belén Faraoni; María Julia Castro; Natalia Paola Alza; Valeria Cavallaro Journal: Curr Neuropharmacol Date: 2013-07 Impact factor: 7.363
Authors: John V Anyam; Priscilla E Daikwo; Marzuq A Ungogo; Nwakaego E Nweze; Ngozichukwuka P Igoli; Alexander I Gray; Harry P De Koning; John O Igoli Journal: Front Chem Date: 2021-04-21 Impact factor: 5.221