BACKGROUND: Hepatocellular carcinoma (HCC) is the most common malignancy in the world, especially in China. Early diagnosis of new and recurrent hepatocellular carcinoma, followed by timely treatment, will help decrease mortality. Currently biomarkers are not satisfactory. Better diagnostic methods are highly demanded. METHODS: In this study, we have used in silico identification and RT-PCR test and discovered a hepatoma associated gene (HTA). Knockdown of endogenous HTA expression was performed by small interfering RNA in malignant hepatocyte HepG2. Then we tested the cell proliferative ability of these cells in vitro and in vivo. RESULTS: HTA was expressed specifically in some kinds of tumors, but not detected in any normal tissues. It was expressed especially high in hepatocellular carcinoma. Knockdown of endogenous HTA expression in HepG2 by small interfering RNA attenuated HCC cell growth. CONCLUSIONS: HCA is a very good marker for tumors, especially for HCC. It could play important roles in HCC development and progression and can be a promising molecular target for the development of new diagnostic and therapeutic strategies for HCC.
BACKGROUND:Hepatocellular carcinoma (HCC) is the most common malignancy in the world, especially in China. Early diagnosis of new and recurrent hepatocellular carcinoma, followed by timely treatment, will help decrease mortality. Currently biomarkers are not satisfactory. Better diagnostic methods are highly demanded. METHODS: In this study, we have used in silico identification and RT-PCR test and discovered a hepatoma associated gene (HTA). Knockdown of endogenous HTA expression was performed by small interfering RNA in malignant hepatocyte HepG2. Then we tested the cell proliferative ability of these cells in vitro and in vivo. RESULTS:HTA was expressed specifically in some kinds of tumors, but not detected in any normal tissues. It was expressed especially high in hepatocellular carcinoma. Knockdown of endogenous HTA expression in HepG2 by small interfering RNA attenuated HCC cell growth. CONCLUSIONS: HCA is a very good marker for tumors, especially for HCC. It could play important roles in HCC development and progression and can be a promising molecular target for the development of new diagnostic and therapeutic strategies for HCC.
Authors: A O Schmitt; T Specht; G Beckmann; E Dahl; C P Pilarsky; B Hinzmann; A Rosenthal Journal: Nucleic Acids Res Date: 1999-11-01 Impact factor: 16.971
Authors: H Okuda; T Nakanishi; K Takatsu; A Saito; N Hayashi; K Takasaki; K Takenami; M Yamamoto; M Nakano Journal: Cancer Date: 2000-02-01 Impact factor: 6.860
Authors: Ahmedin Jemal; Taylor Murray; Elizabeth Ward; Alicia Samuels; Ram C Tiwari; Asma Ghafoor; Eric J Feuer; Michael J Thun Journal: CA Cancer J Clin Date: 2005 Jan-Feb Impact factor: 508.702
Authors: Yan Chen; Christine Miller; Rebecca Mosher; Xumei Zhao; Jim Deeds; Mike Morrissey; Barb Bryant; David Yang; Ron Meyer; Frank Cronin; Bobbie S Gostout; Karen Smith-McCune; Robert Schlegel Journal: Cancer Res Date: 2003-04-15 Impact factor: 12.701
Authors: W T Loging; A Lal; I M Siu; T L Loney; C J Wikstrand; M A Marra; C Prange; D D Bigner; R L Strausberg; G J Riggins Journal: Genome Res Date: 2000-09 Impact factor: 9.043
Authors: Shahrokh F Shariat; Michael W Kattan; Sibel Erdamar; Cuong Nguyen; Peter T Scardino; David M Spencer; Thomas M Wheeler; Kevin M Slawin Journal: J Clin Oncol Date: 2003-04-01 Impact factor: 44.544
Authors: Eduardo M Reis; Elida P B Ojopi; Fernando L Alberto; Paula Rahal; Fernando Tsukumo; Ulises M Mancini; Gustavo S Guimarães; Gloria M A Thompson; Cleber Camacho; Elisabete Miracca; André L Carvalho; Abimael A Machado; Apuã C M Paquola; Janete M Cerutti; Aline M da Silva; Gonçalo G Pereira; Sandro R Valentini; Maria A Nagai; Luiz Paulo Kowalski; Sergio Verjovski-Almeida; Eloiza H Tajara; Emmanuel Dias-Neto; Mario H Bengtson; Renata A Canevari; Marcelo F Carazzolle; Christian Colin; Fernando F Costa; Maria Cristina R Costa; Marcos R H Estécio; Leda Isabel C V Esteves; Miriam H H Federico; Pedro Edson Moreira Guimarães; Christine Hackel; Edna T Kimura; Suzana G Leoni; Ru M B Maciel; Simone Maistro; Flavia R R Mangone; Katlin B Massirer; Silvia E Matsuo; Francisco G Nobrega; Marina Pasetto Nóbrega; Diana Noronha Nunes; Fabio Nunes; José Rodrigo Pandolfi; Maria Inês M C Pardini; Fátima Solange Pasini; Tarcisio Peres; Cláudia Aparecida Rainho; Patrícia P dos Reis; Flávia Cristina C Rodrigus-Lisoni; Silvia Regina Rogatto; Andrey dos Santos; Paulo C C dos Santos; Mar Cleide Sogayar; Cleslei F Zanelli Journal: Cancer Res Date: 2005-03-01 Impact factor: 12.701