Literature DB >> 2012836

Resolution of the conformational distribution and dynamics of a flexible molecule using frequency-domain fluorometry.

J R Lakowicz1, J Kuśba, W Wiczk, I Gryczynski, H Szmacinski, M L Johnson.   

Abstract

We report the first resolution of both the conformational distribution and end-to-end diffusion coefficient of a flexible molecule. This molecular information was recovered using only the donor intensity decay in a single solvent at a single viscosity, as observed by the technique of frequency-domain fluorometry. This technique can be extended to measurements of structural fluctuations of biological macromolecules.

Entities:  

Mesh：

Year:  1991        PMID： 2012836     DOI： 10.1016/0301-4622(91)85008-e

Source DB:  PubMed          Journal:  Biophys Chem        ISSN： 0301-4622            Impact factor:   2.352


  7 in total

1.  Distance distributions and anisotropy decays of troponin C and its complex with troponin I.

Authors:  H C Cheung; C K Wang; I Gryczynski; W Wiczk; G Laczko; M L Johnson; J R Lakowicz
Journal:  Biochemistry       Date:  1991-05-28       Impact factor: 3.162

2.  Distance distributions and dynamics of a zinc finger peptide from fluorescence resonance energy transfer measurements.

Authors:  P S Eis; J Kuśba; M L Johnson; J R Lakowicz
Journal:  J Fluoresc       Date:  1993-03       Impact factor: 2.217

3.  End-to-end diffusion coefficients and distance distributions from fluorescence energy transfer measurements: Enhanced resolution by using multiple donors with different lifetimes.

Authors:  I Gryczynski; J R Lakowicz; J Kuśba
Journal:  J Fluoresc       Date:  1995-06       Impact factor: 2.217

4.  End-to-End Distance Distributions of Flexible Molecules: Frequency-Domain Fluorescence Energy Transfer Measurements and Rotational Isomeric State Model Calculations.

Authors:  Joseph R Lakowicz; Wieslaw Wiczk; Ignacy Gryczynski; Mayer Fishman; Michael L Johnson
Journal:  Macromolecules       Date:  2002-05-01       Impact factor: 5.985

5.  Distance distributions from the tyrosyl to disulfide residues in the oxytocin and [Arg8]-vasopressin measured using frequency-domain fluorescence resonance energy transfer.

Authors:  H Szmacinski; W Wiczk; M N Fishman; P S Eis; J R Lakowicz; M L Johnson
Journal:  Eur Biophys J       Date:  1996       Impact factor: 1.733

6.  Time-resolved energy transfer measurements of donor-acceptor distance distributions and intramolecular flexibility of a CCHH zinc finger peptide.

Authors:  P S Eis; J R Lakowicz
Journal:  Biochemistry       Date:  1993-08-10       Impact factor: 3.162

7.  Selecting Subpopulations of High-Quality Protein Conformers among Conformational Mixtures of Recombinant Bovine MMP-9 Solubilized from Inclusion Bodies.

Authors:  Jose Vicente Carratalá; Laia Gifre-Renom; Ramon Roca-Pinilla; Antonio Villaverde; Anna Arís; Elena Garcia-Fruitós; Julieta María Sánchez; Neus Ferrer-Miralles
Journal:  Int J Mol Sci       Date:  2021-03-16       Impact factor: 5.923
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.