AIM: To evaluate whether crypt abscesses from inflammatory bowel disease (IBD) patients contain bacteria and to establish their nature. METHODS: We studied 17 ulcerative colitis patients, 11 Crohn's disease patients, 7 patients with acute self-limited colitis (ASLC) and normal colonic biopsies from 5 subjects who underwent colonoscopy for colon cancer screening. A fluorescent in situ hybridization technique was applied to colonic biopsies to assess the microbiota composition of the crypts and crypt abscesses. RESULTS: Crypts colonized by bacteria were observed in 42.9% and 3.6% of ASLC and IBD patients, respectively (P = 0.019). Crypt abscesses colonized by bacteria were observed in 28.6% and 0.0% of ASLC and IBD patients, respectively (P = 0.035). CONCLUSION: These results do not support the hypothesis that crypt abscesses in IBD are the result of localized dysbiosis arising from persistence of living bacteria colonizing the crypts.
AIM: To evaluate whether crypt abscesses from inflammatory bowel disease (IBD) patients contain bacteria and to establish their nature. METHODS: We studied 17 ulcerative colitispatients, 11 Crohn's diseasepatients, 7 patients with acute self-limited colitis (ASLC) and normal colonic biopsies from 5 subjects who underwent colonoscopy for colon cancer screening. A fluorescent in situ hybridization technique was applied to colonic biopsies to assess the microbiota composition of the crypts and crypt abscesses. RESULTS: Crypts colonized by bacteria were observed in 42.9% and 3.6% of ASLC and IBDpatients, respectively (P = 0.019). Crypt abscesses colonized by bacteria were observed in 28.6% and 0.0% of ASLC and IBDpatients, respectively (P = 0.035). CONCLUSION: These results do not support the hypothesis that crypt abscesses in IBD are the result of localized dysbiosis arising from persistence of living bacteria colonizing the crypts.
Authors: P Seksik; H Sokol; P Lepage; N Vasquez; C Manichanh; I Mangin; P Pochart; J Doré; P Marteau Journal: Aliment Pharmacol Ther Date: 2006-10 Impact factor: 8.171
Authors: Gamze Ayata; Sarathehandra Ithamukkala; Heidi Sapp; Beth H Shaz; Tom P Brien; Helen H Wang; Donald A Antonioli; Francis A Farraye; Robert D Odze Journal: Am J Surg Pathol Date: 2002-11 Impact factor: 6.394
Authors: Henri Braat; Peter McGuirk; Fiebo J W Ten Kate; Inge Huibregtse; Padraic J Dunne; Daan W Hommes; Sander J H Van Deventer; Kingston H G Mills Journal: Gut Date: 2006-09-04 Impact factor: 23.059
Authors: Jan Wehkamp; Juergen Harder; Michael Weichenthal; Oliver Mueller; Klaus R Herrlinger; Klaus Fellermann; Jens M Schroeder; Eduard F Stange Journal: Inflamm Bowel Dis Date: 2003-07 Impact factor: 5.325