Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Crystallization and preliminary X-ray diffraction analysis of salutaridine reductase from the opium poppy Papaver somniferum.

Literature DB >> 20124713

Crystallization and preliminary X-ray diffraction analysis of salutaridine reductase from the opium poppy Papaver somniferum.

Yasuhiro Higashi¹, Thomas J Smith, Joseph M Jez, Toni M Kutchan.

Abstract

The opium poppy Papaver somniferum is the source of the narcotic analgesics morphine and codeine. Salutaridine reductase (SalR; EC 1.1.1.248) reduces the C-7 keto group of salutaridine to the C-7 (S)-hydroxyl group of salutaridinol in the biosynthetic pathway that leads to morphine in the opium poppy plant. P. somniferum SalR was overproduced in Escherichia coli and purified using cobalt-affinity and size-exclusion chromatography. Hexagonal crystals belonging to space group P6(4)22 or P6(2)22 were obtained using ammonium sulfate as precipitant and diffracted to a resolution of 1.9 A.

Entities: Chemical Species

Mesh：

Substances：

Year: 2010 PMID： 20124713 PMCID： PMC2815683 DOI： 10.1107/S174430910904932X

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

16 in total

1. Porcine carbonyl reductase. structural basis for a functional monomer in short chain dehydrogenases/reductases.

Authors: D Ghosh; M Sawicki; V Pletnev; M Erman; S Ohno; S Nakajin; W L Duax
Journal: J Biol Chem Date: 2001-03-08 Impact factor: 5.157

2. Identification of the reactive cysteine residue (Cys227) in human carbonyl reductase.

Authors: J N Tinguely; B Wermuth
Journal: Eur J Biochem Date: 1999-02

3. Matthews coefficient probabilities: Improved estimates for unit cell contents of proteins, DNA, and protein-nucleic acid complex crystals.

Authors: Katherine A Kantardjieff; Bernhard Rupp
Journal: Protein Sci Date: 2003-09 Impact factor: 6.725

4. INVESTIGATIONS ON THE BIOSYNTHESIS OF MORPHINE ALKALOIDS.

Authors: D H BARTON; G W KIRBY; W STEGLICH; G M THOMAS; A R BATTERSBY; T A DOBSON; H RAMUZ
Journal: J Chem Soc Date: 1965-04

5. RNAi suppression of the morphine biosynthetic gene salAT and evidence of association of pathway enzymes.

Authors: Katja Kempe; Yasuhiro Higashi; Susanne Frick; Khaled Sabarna; Toni M Kutchan
Journal: Phytochemistry Date: 2009-04-07 Impact factor: 4.072

6. Structure of tropinone reductase-II complexed with NADP+ and pseudotropine at 1.9 A resolution: implication for stereospecific substrate binding and catalysis.

Authors: A Yamashita; H Kato; S Wakatsuki; T Tomizaki; T Nakatsu; K Nakajima; T Hashimoto; Y Yamada; J Oda
Journal: Biochemistry Date: 1999-06-15 Impact factor: 3.162

3. Cloning and characterization of a norbelladine 4'-O-methyltransferase involved in the biosynthesis of the Alzheimer's drug galanthamine in Narcissus sp. aff. pseudonarcissus.

Authors: Matthew B Kilgore; Megan M Augustin; Courtney M Starks; Mark O'Neil-Johnson; Gregory D May; John A Crow; Toni M Kutchan
Journal: PLoS One Date: 2014-07-25 Impact factor: 3.240

3 in total

Crystallization and preliminary X-ray diffraction analysis of salutaridine reductase from the opium poppy Papaver somniferum.

1. Porcine carbonyl reductase. structural basis for a functional monomer in short chain dehydrogenases/reductases.

2. Identification of the reactive cysteine residue (Cys227) in human carbonyl reductase.

3. Matthews coefficient probabilities: Improved estimates for unit cell contents of proteins, DNA, and protein-nucleic acid complex crystals.

4. INVESTIGATIONS ON THE BIOSYNTHESIS OF MORPHINE ALKALOIDS.

5. RNAi suppression of the morphine biosynthetic gene salAT and evidence of association of pathway enzymes.

6. Structure of tropinone reductase-II complexed with NADP+ and pseudotropine at 1.9 A resolution: implication for stereospecific substrate binding and catalysis.

7. Short-chain dehydrogenases/reductases (SDRs).

Review 8. Short-chain dehydrogenases/reductases (SDR).

9. Structural analysis of actinorhodin polyketide ketoreductase: cofactor binding and substrate specificity.

10. An unbiased cell morphology-based screen for new, biologically active small molecules.

Review 1. Yeast factories for the production of aromatic compounds: from building blocks to plant secondary metabolites.

2. Atomic structure of salutaridine reductase from the opium poppy (Papaver somniferum).

3. Cloning and characterization of a norbelladine 4'-O-methyltransferase involved in the biosynthesis of the Alzheimer's drug galanthamine in Narcissus sp. aff. pseudonarcissus.