Literature DB >> 20111982

Screening of DNA aptamer which binds to alpha-synuclein.

Kaori Tsukakoshi1, Ryuichi Harada, Koji Sode, Kazunori Ikebukuro.   

Abstract

Alpha-synuclein is a native, unfolded protein that causes several neurodegenerative diseases such as dementia with Lewy bodies and Parkinson's disease. We have now identified the first DNA aptamers against alpha-synuclein using native PAGE applied to the SELEX method. We call this aptamer "M5-15"; it is the alpha-synuclein-bound aptamer and was isolated after four cycles of screening. M5-15 is composed of three stem-loop structures that may play an important role in the binding to alpha-synuclein. Moreover, M5-15 specifically binds to the alpha-synuclein monomer and oligomer. We expect that this aptamer will become a useful tool in alpha-synuclein analysis and diagnosis.

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Year:  2010        PMID： 20111982     DOI： 10.1007/s10529-010-0200-5

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  12 in total

1.  Inhibition of Aggregation of Mutant Huntingtin by Nucleic Acid Aptamers In Vitro and in a Yeast Model of Huntington's Disease.

Authors:  Rajeev K Chaudhary; Kinjal A Patel; Milan K Patel; Radha H Joshi; Ipsita Roy
Journal:  Mol Ther       Date:  2015-08-27       Impact factor: 11.454

Review 2.  Trends in the Design and Development of Specific Aptamers Against Peptides and Proteins.

Authors:  Maryam Tabarzad; Marzieh Jafari
Journal:  Protein J       Date:  2016-04       Impact factor: 2.371

Review 3.  Development of Polymeric Nanoparticles for Blood-Brain Barrier Transfer-Strategies and Challenges.

Authors:  Weisen Zhang; Ami Mehta; Ziqiu Tong; Lars Esser; Nicolas H Voelcker
Journal:  Adv Sci (Weinh)       Date:  2021-03-07       Impact factor: 16.806

4.  Species-barrier on the cross-species oral transmission of bovine AA amyloidosis in mice.

Authors:  Susumu Iwaide; Naoki Ujike; Kyoko Kobayashi; Yukiko Sassa; Tomoaki Murakami
Journal:  J Vet Med Sci       Date:  2021-04-28       Impact factor: 1.267

Review 5.  Nucleic Acid Aptamers: Emerging Applications in Medical Imaging, Nanotechnology, Neurosciences, and Drug Delivery.

Authors:  Pascal Röthlisberger; Cécile Gasse; Marcel Hollenstein
Journal:  Int J Mol Sci       Date:  2017-11-16       Impact factor: 5.923

Review 6.  Aptamers Selected for Recognizing Amyloid β-Protein-A Case for Cautious Optimism.

Authors:  Farid Rahimi
Journal:  Int J Mol Sci       Date:  2018-02-27       Impact factor: 5.923

Review 7.  Synucleins and Gene Expression: Ramblers in a Crowd or Cops Regulating Traffic?

Authors:  Alexei A Surguchev; Andrei Surguchov
Journal:  Front Mol Neurosci       Date:  2017-07-13       Impact factor: 5.639

8.  Novel DNA Aptamers for Parkinson's Disease Treatment Inhibit α-Synuclein Aggregation and Facilitate its Degradation.

Authors:  Yuan Zheng; Jing Qu; Fenqin Xue; Yan Zheng; Bo Yang; Yongchang Chang; Hui Yang; Jianliang Zhang
Journal:  Mol Ther Nucleic Acids       Date:  2018-03-06       Impact factor: 8.886

9.  High-Affinity Detection of Alpha-Synuclein by Aptamer-Gold Conjugates on an Amine-Modified Dielectric Surface.

Authors:  Xuemei You; Subash C B Gopinath; Thangavel Lakshmipriya; Dingan Li
Journal:  J Anal Methods Chem       Date:  2019-11-30       Impact factor: 2.193

Review 10.  Progress in the molecular pathogenesis and nucleic acid therapeutics for Parkinson's disease in the precision medicine era.

Authors:  Dunhui Li; Frank L Mastaglia; Sue Fletcher; Steve D Wilton
Journal:  Med Res Rev       Date:  2020-08-06       Impact factor: 12.944
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