| Literature DB >> 20093760 |
Ben Hatano1, Takayuki Maki, Takeyuki Obara, Hitomi Fukumoto, Kohsuke Hagisawa, Yoshitaro Matsushita, Akiko Okutani, Boldbaastar Bazartseren, Satoshi Inoue, Tetsutaro Sata, Harutaka Katano.
Abstract
A quick, reliable detection system is necessary to deal with bioterrorism. Loop-mediated isothermal amplification (LAMP) is a DNA amplification method that can amplify specific DNA fragments in isothermal conditions. We developed a new highly mobile and practical LAMP anthrax detection system that uses a disposable pocket warmer without the need for electricity (pocket-warmer LAMP). In our tests, the detection limit of the pocket-warmer LAMP was 1,000 copies of Bacillus anthracis pag and capB gene fragments per tube. The pocket-warmer LAMP also detected B. anthracis genes from DNA extracted from 0.1 volume of a B. anthracis colony. The lower detection limit of the pocket-warmer LAMP was not significantly different from that of a conventional LAMP using a heat block, and was not changed under cold (4 degrees C) or warm (37 degrees C) conditions in a Styrofoam box. The pocket-warmer LAMP could be useful against bioterrorism, and as a sensitive, reliable detection tool in areas with undependable electricity infrastructures.Entities:
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Year: 2010 PMID： 20093760
Source DB: PubMed Journal: Jpn J Infect Dis ISSN： 1344-6304 Impact factor: 1.362