Paul R Cook1, Christine Glenn, Annie Armston. 1. Southampton University Hospitals NHS Trust, Clinical Pathology Care Group, Division of Diagnostics & Therapy, Clinical Biochemistry, Level D, South Block, Mailpoint 6, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, United Kingdom. paul.cook@suht.swest.nhs.uk
Abstract
OBJECTIVES: To evaluate the effect of hemolysis on the measurement of insulin using the Beckman Coulter Unicell DXI 800 immunoassay analyzer. DESIGN AND METHODS: Insulin concentrations were measured using the Beckman Coulter Unicell DXI 800. Interference studies were conducted by mixing plasma of known insulin concentrations with hemolysates of increasing hemoglobin concentration. Hemolysates were prepared by osmotic shock. A change of >10% from baseline results was taken as evidence of significant interference. RESULTS: Hemolysates mediated a concentration-dependent reduction in insulin concentration. CONCLUSIONS: A concentration-dependent negative bias in the measurement of insulin is associated with increasing degrees of hemolysis on the Beckman Coulter Unicell DXI 800. Copyright 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
OBJECTIVES: To evaluate the effect of hemolysis on the measurement of insulin using the Beckman Coulter Unicell DXI 800 immunoassay analyzer. DESIGN AND METHODS: Insulin concentrations were measured using the Beckman Coulter Unicell DXI 800. Interference studies were conducted by mixing plasma of known insulin concentrations with hemolysates of increasing hemoglobin concentration. Hemolysates were prepared by osmotic shock. A change of >10% from baseline results was taken as evidence of significant interference. RESULTS: Hemolysates mediated a concentration-dependent reduction in insulin concentration. CONCLUSIONS: A concentration-dependent negative bias in the measurement of insulin is associated with increasing degrees of hemolysis on the Beckman Coulter Unicell DXI 800. Copyright 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
Authors: Julia Steinberger; Stephen R Daniels; Nancy Hagberg; Carmen R Isasi; Aaron S Kelly; Donald Lloyd-Jones; Russell R Pate; Charlotte Pratt; Christina M Shay; Jeffrey A Towbin; Elaine Urbina; Linda V Van Horn; Justin P Zachariah Journal: Circulation Date: 2016-08-11 Impact factor: 29.690
Authors: Andrew P Demidowich; Jordan A Levine; Sheila M Brady; Cheryl D Johnson; Steven J Soldin; Jack A Yanovski Journal: Diabetes Obes Metab Date: 2020-04-20 Impact factor: 6.408