BACKGROUND AND PURPOSE: The subplate layer and intermediate zone are the precursors for neonatal white matter. The aims of this study were to evaluate 1) T1 and T2 signal intensity, and 2) FA of subplate and intermediate zone in postmortem fetuses and correlate with histology, and 3) T2 signal intensity of subplate and intermediate zone on antenatal MR imaging. MATERIALS AND METHODS: Fourteen immersion-fixed normal brains from 18 to 25 gestational weeks underwent 1.5T MR imaging, including DTI and histologic examination. The subplate and intermediate zone were graded on a scale of 1-5 on T1 and T2, and FAs were evaluated and then correlated with age. Seventeen antenatal MR images from 20 to 26 gestational weeks with normal brain were evaluated by using the same grading. RESULTS: On T1 postmortem MR imaging, subplate has lower signal intensity compared with intermediate zone; subplate signal intensity correlated positively (r = 0.66, P = .012) with age, and intermediate zone signal intensity correlated negatively (r = -0.78, P = .001) with age. On T2 postmortem MR imaging, subplate has higher signal intensity compared with intermediate zone and remained persistently high in signal intensity; intermediate zone signal intensity showed moderate correlation (r = 0.48, P = .086) with age. FA of subplate correlated positively (r = 0.55, P < .001) with age; FA of intermediate zone correlated negatively (r = -0.64, P < .0001) with age. On histology, extracellular matrix decreased and cellularity increased in subplate layer, tangentially organized cellularity decreased, and projecting fibers became thicker in intermediate zone with increasing gestation. The findings on T2-weighted antenatal MR imaging were similar to T2-weighted postmortem MR imaging. CONCLUSIONS: The changes in signal intensity and FA of subplate and intermediate zone in the second trimester reflect microstructural changes on histology.
BACKGROUND AND PURPOSE: The subplate layer and intermediate zone are the precursors for neonatal white matter. The aims of this study were to evaluate 1) T1 and T2 signal intensity, and 2) FA of subplate and intermediate zone in postmortem fetuses and correlate with histology, and 3) T2 signal intensity of subplate and intermediate zone on antenatal MR imaging. MATERIALS AND METHODS: Fourteen immersion-fixed normal brains from 18 to 25 gestational weeks underwent 1.5T MR imaging, including DTI and histologic examination. The subplate and intermediate zone were graded on a scale of 1-5 on T1 and T2, and FAs were evaluated and then correlated with age. Seventeen antenatal MR images from 20 to 26 gestational weeks with normal brain were evaluated by using the same grading. RESULTS: On T1 postmortem MR imaging, subplate has lower signal intensity compared with intermediate zone; subplate signal intensity correlated positively (r = 0.66, P = .012) with age, and intermediate zone signal intensity correlated negatively (r = -0.78, P = .001) with age. On T2 postmortem MR imaging, subplate has higher signal intensity compared with intermediate zone and remained persistently high in signal intensity; intermediate zone signal intensity showed moderate correlation (r = 0.48, P = .086) with age. FA of subplate correlated positively (r = 0.55, P < .001) with age; FA of intermediate zone correlated negatively (r = -0.64, P < .0001) with age. On histology, extracellular matrix decreased and cellularity increased in subplate layer, tangentially organized cellularity decreased, and projecting fibers became thicker in intermediate zone with increasing gestation. The findings on T2-weighted antenatal MR imaging were similar to T2-weighted postmortem MR imaging. CONCLUSIONS: The changes in signal intensity and FA of subplate and intermediate zone in the second trimester reflect microstructural changes on histology.
Authors: Bernard Metz; Gideon F A Kersten; Peter Hoogerhout; Humphrey F Brugghe; Hans A M Timmermans; Ad de Jong; Hugo Meiring; Jan ten Hove; Wim E Hennink; Daan J A Crommelin; Wim Jiskoot Journal: J Biol Chem Date: 2003-11-24 Impact factor: 5.157
Authors: Luis C Maas; Pratik Mukherjee; Julio Carballido-Gamio; Srivathsa Veeraraghavan; Steven P Miller; Savannah C Partridge; Roland G Henry; A James Barkovich; Daniel B Vigneron Journal: Neuroimage Date: 2004-07 Impact factor: 6.556
Authors: Vidya Rajagopalan; Julia Scott; Piotr A Habas; Kio Kim; James Corbett-Detig; Francois Rousseau; A James Barkovich; Orit A Glenn; Colin Studholme Journal: J Neurosci Date: 2011-02-23 Impact factor: 6.167
Authors: Adrian Viehweger; Till Riffert; Bibek Dhital; Thomas R Knösche; Alfred Anwander; Holger Stepan; Ina Sorge; Wolfgang Hirsch Journal: Pediatr Radiol Date: 2014-05-10
Authors: Julia A Scott; Piotr A Habas; Kio Kim; Vidya Rajagopalan; Kia S Hamzelou; James M Corbett-Detig; A James Barkovich; Orit A Glenn; Colin Studholme Journal: Int J Dev Neurosci Date: 2011-04-17 Impact factor: 2.457
Authors: Lana Vasung; Esra Abaci Turk; Silvina L Ferradal; Jason Sutin; Jeffrey N Stout; Banu Ahtam; Pei-Yi Lin; P Ellen Grant Journal: Neuroimage Date: 2018-07-21 Impact factor: 6.556
Authors: J Corbett-Detig; P A Habas; J A Scott; K Kim; V Rajagopalan; P S McQuillen; A J Barkovich; O A Glenn; C Studholme Journal: Brain Struct Funct Date: 2010-10-29 Impact factor: 3.270
Authors: Christian Mitter; András Jakab; Peter C Brugger; Gerda Ricken; Gerlinde M Gruber; Dieter Bettelheim; Anke Scharrer; Georg Langs; Johannes A Hainfellner; Daniela Prayer; Gregor Kasprian Journal: Front Neuroanat Date: 2015-12-24 Impact factor: 3.856
Authors: Lana Vasung; Claude Lepage; Milan Radoš; Mihovil Pletikos; Jennifer S Goldman; Jonas Richiardi; Marina Raguž; Elda Fischi-Gómez; Sherif Karama; Petra S Huppi; Alan C Evans; Ivica Kostovic Journal: Front Neuroanat Date: 2016-02-24 Impact factor: 3.856