OBJECTIVES: In recent years, obesity has become a global epidemic, highlighting the necessity for basic research into mechanisms underlying growth of adipose tissue and differentiation of stem cells into adipocytes, in humans. For better understanding of cell signalling in adipogenesis, the role of DNER (delta/Notch-like EGF-related receptor) in adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAMSC) was investigated. MATERIALS AND METHODS: To assess the role of DNER in hAMSC adipogenesis, hAMSCs were transfected with DNER small interfering RNA (siDNER). Real-time quantitative reverse transcriptase polymerase chain reactions to assess expression levels of adipogenesis-related genes regulated by siDNER, cell cycle and immunoblot analyses were performed. RESULTS: First, it was determined that DNER mRNA was profoundly expressed in hAMSCs and reduced during adipogenic differentiation. Knockdown of DNER altered cell morphology, inhibited proliferation and increased frequency and efficiency of adipogenesis in hAMSC. Expression of CCAAT/enhancer-binding protein delta increased and proportion of cells in S phase decreased by knockdown of DNER, using specific siRNA. Moreover, adipocyte-specific genes including peroxisome proliferator-activated receptor gamma, fatty acid binding protein 4 and perilipin were up-regulated in siDNER compared to the siControl group during adipogenesis in hAMSC. CONCLUSIONS: These results indicate that DNER knockdown in hAMSC accelerated onset of adipogenic differentiation by bypassing mitotic clonal expansion during the early stages of adipogenesis.
OBJECTIVES: In recent years, obesity has become a global epidemic, highlighting the necessity for basic research into mechanisms underlying growth of adipose tissue and differentiation of stem cells into adipocytes, in humans. For better understanding of cell signalling in adipogenesis, the role of DNER (delta/Notch-like EGF-related receptor) in adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAMSC) was investigated. MATERIALS AND METHODS: To assess the role of DNER in hAMSC adipogenesis, hAMSCs were transfected with DNER small interfering RNA (siDNER). Real-time quantitative reverse transcriptase polymerase chain reactions to assess expression levels of adipogenesis-related genes regulated by siDNER, cell cycle and immunoblot analyses were performed. RESULTS: First, it was determined that DNER mRNA was profoundly expressed in hAMSCs and reduced during adipogenic differentiation. Knockdown of DNER altered cell morphology, inhibited proliferation and increased frequency and efficiency of adipogenesis in hAMSC. Expression of CCAAT/enhancer-binding protein delta increased and proportion of cells in S phase decreased by knockdown of DNER, using specific siRNA. Moreover, adipocyte-specific genes including peroxisome proliferator-activated receptor gamma, fatty acid binding protein 4 and perilipin were up-regulated in siDNER compared to the siControl group during adipogenesis in hAMSC. CONCLUSIONS: These results indicate that DNER knockdown in hAMSC accelerated onset of adipogenic differentiation by bypassing mitotic clonal expansion during the early stages of adipogenesis.
Authors: Rebecca Hein; Dieter Flesch-Janys; Norbert Dahmen; Lars Beckmann; Sara Lindström; Nils Schoof; Kamila Czene; Kirstin Mittelstraß; Thomas Illig; Petra Seibold; Sabine Behrens; Keith Humphreys; Jingmei Li; Jianjun Liu; Janet E Olson; Xianshu Wang; Susan E Hankinson; Thérèse Truong; Florence Menegaux; Isabel Dos Santos Silva; Nichola Johnson; Shou-Tung Chen; Jyh-Cherng Yu; Argyrios Ziogas; Vesa Kataja; Veli-Matti Kosma; Arto Mannermaa; Hoda Anton-Culver; Chen-Yang Shen; Hiltrud Brauch; Julian Peto; Pascal Guénel; Peter Kraft; Fergus J Couch; Douglas F Easton; Per Hall; Jenny Chang-Claude Journal: Breast Cancer Res Treat Date: 2013-02-20 Impact factor: 4.872
Authors: Dana B Hancock; María Soler Artigas; Sina A Gharib; Amanda Henry; Ani Manichaikul; Adaikalavan Ramasamy; Daan W Loth; Medea Imboden; Beate Koch; Wendy L McArdle; Albert V Smith; Joanna Smolonska; Akshay Sood; Wenbo Tang; Jemma B Wilk; Guangju Zhai; Jing Hua Zhao; Hugues Aschard; Kristin M Burkart; Ivan Curjuric; Mark Eijgelsheim; Paul Elliott; Xiangjun Gu; Tamara B Harris; Christer Janson; Georg Homuth; Pirro G Hysi; Jason Z Liu; Laura R Loehr; Kurt Lohman; Ruth J F Loos; Alisa K Manning; Kristin D Marciante; Ma'en Obeidat; Dirkje S Postma; Melinda C Aldrich; Guy G Brusselle; Ting-hsu Chen; Gudny Eiriksdottir; Nora Franceschini; Joachim Heinrich; Jerome I Rotter; Cisca Wijmenga; O Dale Williams; Amy R Bentley; Albert Hofman; Cathy C Laurie; Thomas Lumley; Alanna C Morrison; Bonnie R Joubert; Fernando Rivadeneira; David J Couper; Stephen B Kritchevsky; Yongmei Liu; Matthias Wjst; Louise V Wain; Judith M Vonk; André G Uitterlinden; Thierry Rochat; Stephen S Rich; Bruce M Psaty; George T O'Connor; Kari E North; Daniel B Mirel; Bernd Meibohm; Lenore J Launer; Kay-Tee Khaw; Anna-Liisa Hartikainen; Christopher J Hammond; Sven Gläser; Jonathan Marchini; Peter Kraft; Nicholas J Wareham; Henry Völzke; Bruno H C Stricker; Timothy D Spector; Nicole M Probst-Hensch; Deborah Jarvis; Marjo-Riitta Jarvelin; Susan R Heckbert; Vilmundur Gudnason; H Marike Boezen; R Graham Barr; Patricia A Cassano; David P Strachan; Myriam Fornage; Ian P Hall; Josée Dupuis; Martin D Tobin; Stephanie J London Journal: PLoS Genet Date: 2012-12-20 Impact factor: 5.917
Authors: Johnny Lam; Ian H Bellayr; Ross A Marklein; Steven R Bauer; Raj K Puri; Kyung E Sung Journal: Stem Cells Transl Med Date: 2018-08-07 Impact factor: 6.940