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Literature DB >> 20062887

Chemical rescue of protein tyrosine phosphatase activity.

Abstract

We report that the activity of a rationally engineered protein tyrosine phosphatase (PTP) mutant can be fully rescued by the addition of the biarsenical fluorescein derivative FlAsH, a compound that does not affect the activity of wild-type PTPs.

Entities: Chemical Disease Gene Species

Mesh：

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Year: 2009 PMID： 20062887 PMCID： PMC2834275 DOI： 10.1039/b919815f

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

20 in total

1. Fluorescent labeling of recombinant proteins in living cells with FlAsH.

Authors: B A Griffin; S R Adams; J Jones; R Y Tsien
Journal: Methods Enzymol Date: 2000 Impact factor: 1.600

Review 2. Structural and evolutionary relationships among protein tyrosine phosphatase domains.

Authors: J N Andersen; O H Mortensen; G H Peters; P G Drake; L F Iversen; O H Olsen; P G Jansen; H S Andersen; N K Tonks; N P Møller
Journal: Mol Cell Biol Date: 2001-11 Impact factor: 4.272

Review 3. Protein tyrosine phosphatases: from genes, to function, to disease.

Authors: Nicholas K Tonks
Journal: Nat Rev Mol Cell Biol Date: 2006-11 Impact factor: 94.444

4. Ligand interaction scan: a general method for engineering ligand-sensitive protein alleles.

Authors: Oran Erster; Miriam Eisenstein; Mordechai Liscovitch
Journal: Nat Methods Date: 2007-04-22 Impact factor: 28.547

5. Chemical rescue of a mutant enzyme in living cells.

Authors: Yingfeng Qiao; Henrik Molina; Akhilesh Pandey; Jin Zhang; Philip A Cole
Journal: Science Date: 2006-03-03 Impact factor: 47.728

6. Specific covalent labeling of recombinant protein molecules inside live cells.

Authors: B A Griffin; S R Adams; R Y Tsien
Journal: Science Date: 1998-07-10 Impact factor: 47.728

7. Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST.

Authors: A J Garton; N K Tonks
Journal: J Biol Chem Date: 1999-02-05 Impact factor: 5.157

8. The protein-labeling reagent FLASH-EDT2 binds not only to CCXXCC motifs but also non-specifically to endogenous cysteine-rich proteins.

Authors: K Stroffekova; C Proenza; K G Beam
Journal: Pflugers Arch Date: 2001-09 Impact factor: 3.657

Chemical rescue of protein tyrosine phosphatase activity.

1. Fluorescent labeling of recombinant proteins in living cells with FlAsH.

Review 2. Structural and evolutionary relationships among protein tyrosine phosphatase domains.

Review 3. Protein tyrosine phosphatases: from genes, to function, to disease.

4. Ligand interaction scan: a general method for engineering ligand-sensitive protein alleles.

5. Chemical rescue of a mutant enzyme in living cells.

6. Specific covalent labeling of recombinant protein molecules inside live cells.

7. Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST.

8. The protein-labeling reagent FLASH-EDT2 binds not only to CCXXCC motifs but also non-specifically to endogenous cysteine-rich proteins.

Review 9. Protein tyrosine phosphatases: emerging regulators of apoptosis.

10. Hairpin structure of a biarsenical-tetracysteine motif determined by NMR spectroscopy.

1. Specific inhibition of sensitized protein tyrosine phosphatase 1B (PTP1B) with a biarsenical probe.

2. Rotamer-Restricted Fluorogenicity of the Bis-Arsenical ReAsH.

3. Direct Chemical Activation of a Rationally Engineered Signaling Enzyme.

4. Activation of Engineered Protein Tyrosine Phosphatases with the Biarsenical Compound AsCy3-EDT₂.

5. Chemical activation of divergent protein tyrosine phosphatase domains with cyanine-based biarsenicals.