Literature DB >> 12010029

High-efficiency fluorescence quenching of conjugated polymers by proteins.

Chunhai Fan1, Kevin W Plaxco, Alan J Heeger.   

Abstract

The fluorescence of the water-soluble anionic conjugated polymer, poly[lithium 5-methoxy-2-(4-sulfobutoxy)-1,4-phenylenevinylene] (MBL-PPV), is quenched in dilute aqueous solution by cytochrome c, a small, naturally occurring electron-transfer protein. The large value obtained for the Stern-Volmer constant (K(sv) = 3.2 x 10(8) at pH 7.4, and approximately 10(9) in acidic solutions) is attributed to a combination of two factors: (1) facile ET between the luminescent semiconducting polymer and the protein and (2) the Columb attraction between the oppositely charged polyelectrolytes. This system shows significant potential for biosensor applications.

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Year:  2002        PMID： 12010029     DOI： 10.1021/ja025899u

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  14 in total

1.  Selective sensing of metalloproteins from nonselective binding using a fluorogenic amphiphilic polymer.

Authors:  Britto S Sandanaraj; Robert Demont; Sivakumar V Aathimanikandan; Elamprakash N Savariar; S Thayumanavan
Journal:  J Am Chem Soc       Date:  2006-08-23       Impact factor: 15.419

2.  A photoactive semisynthetic metalloenzyme exhibits complete selectivity for CO2 reduction in water.

Authors:  Camille R Schneider; Anastasia C Manesis; Michael J Stevenson; Hannah S Shafaat
Journal:  Chem Commun (Camb)       Date:  2018-05-03       Impact factor: 6.222

Review 3.  Recent developments in the synthesis of regioregular thiophene-based conjugated polymers for electronic and optoelectronic applications using nickel and palladium-based catalytic systems.

Authors:  Bibi Amna; Humaira Masood Siddiqi; Abbas Hassan; Turan Ozturk
Journal:  RSC Adv       Date:  2020-01-27       Impact factor: 4.036

4.  Poly(styrene-4-sulfonate)-protected copper nanoclusters as a fluorometric probe for sequential detection of cytochrome c and trypsin.

Authors:  Yanling Hu; Yu He; Yaxue Han; Yili Ge; Gongwu Song; Jiangang Zhou
Journal:  Mikrochim Acta       Date:  2018-07-21       Impact factor: 5.833

5.  On the binding of cationic, water-soluble conjugated polymers to DNA: electrostatic and hydrophobic interactions.

Authors:  Fan Xia; Xiaolei Zuo; Renqiang Yang; Yi Xiao; Di Kang; Alexis Vallée-Bélisle; Xiong Gong; Alan J Heeger; Kevin W Plaxco
Journal:  J Am Chem Soc       Date:  2010-02-03       Impact factor: 15.419

6.  Efficient fluorescent recognition of carboxylates in aqueous media using facilely electrosynthesized poly(9-aminofluorene).

Authors:  Ge Zhang; Yangping Wen; Yuzhen Li; Jingkun Xu; Chaoqun Guo; Baoyang Lu; Danhua Zhu
Journal:  J Fluoresc       Date:  2013-05-29       Impact factor: 2.217

7.  Amplified fluorescence sensing of protease activity with conjugated polyelectrolytes.

Authors:  Mauricio R Pinto; Kirk S Schanze
Journal:  Proc Natl Acad Sci U S A       Date:  2004-05-10       Impact factor: 11.205

8.  Beyond superquenching: hyper-efficient energy transfer from conjugated polymers to gold nanoparticles.

Authors:  Chunhai Fan; Shu Wang; Janice W Hong; Guillermo C Bazan; Kevin W Plaxco; Alan J Heeger
Journal:  Proc Natl Acad Sci U S A       Date:  2003-05-15       Impact factor: 11.205

9.  Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: the role of electrostatic and hydrophobic interactions.

Authors:  Qing-Hua Xu; Brent S Gaylord; Shu Wang; Guillermo C Bazan; Daniel Moses; Alan J Heeger
Journal:  Proc Natl Acad Sci U S A       Date:  2004-07-28       Impact factor: 11.205

10.  Magnetically assisted DNA assays: high selectivity using conjugated polymers for amplified fluorescent transduction.

Authors:  Hui Xu; Haiping Wu; Fei Huang; Shiping Song; Wenxin Li; Yong Cao; Chunhai Fan
Journal:  Nucleic Acids Res       Date:  2005-05-19       Impact factor: 16.971
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