Literature DB >> 2003918

Producing single-stranded DNA probes with the Taq DNA polymerase: a high yield protocol.

U Finckh1, P A Lingenfelter, D Myerson.   

Abstract

We report an efficient procedure to synthesize either single- or double-stranded probes labeled with biotin-11-dUTP, biotin-21-dUTP or digoxigenin-11-dUTP. To produce the single-stranded probe, only a single primer is utilized in a Taq polymerase amplification of 55 cycles. A cytomegalovirus probe is presented. This procedure allows easy production of nonradioactively labeled pure single-stranded probes of any desired length and specificity.

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Year:  1991        PMID： 2003918

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  23 in total

1.  Molecular characterization of a novel gene family encoding ACT domain repeat proteins in Arabidopsis.

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Review 2.  Molecular biology made easy. The polymerase chain reaction.

Authors:  A M Clarke; N P Mapstone; P Quirke
Journal:  Histochem J       Date:  1992-12

3.  An optimized PCR leads to rapid and highly sensitive detection of Borrelia burgdorferi in patients with Lyme borreliosis.

Authors:  S Priem; M G Rittig; T Kamradt; G R Burmester; A Krause
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948

4.  Arabidopsis gls mutants and distinct Fd-GOGAT genes. Implications for photorespiration and primary nitrogen assimilation.

Authors:  K T Coschigano; R Melo-Oliveira; J Lim; G M Coruzzi
Journal:  Plant Cell       Date:  1998-05       Impact factor: 11.277

5.  Panfungal PCR assay for detection of fungal infection in human blood specimens.

Authors:  J A Van Burik; D Myerson; R W Schreckhise; R A Bowden
Journal:  J Clin Microbiol       Date:  1998-05       Impact factor: 5.948

6.  Transcriptional analysis of the principal cell division gene, ftsZ, of Mycobacterium tuberculosis.

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7.  Site-directed mutagenesis by double polymerase chain reaction.

Authors:  S Barik
Journal:  Mol Biotechnol       Date:  1995-02       Impact factor: 2.695

8.  Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. II. High-density labeling of natural DNA.

Authors:  Taurai Tasara; Bernhard Angerer; Martine Damond; Holger Winter; Sabine Dörhöfer; Ulrich Hübscher; Mario Amacker
Journal:  Nucleic Acids Res       Date:  2003-05-15       Impact factor: 16.971

9.  Arabidopsis mutant analysis and gene regulation define a nonredundant role for glutamate dehydrogenase in nitrogen assimilation.

Authors:  R Melo-Oliveira; I C Oliveira; G M Coruzzi
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-14       Impact factor: 11.205

10.  Chloroplast localization of methylerythritol 4-phosphate pathway enzymes and regulation of mitochondrial genes in ispD and ispE albino mutants in Arabidopsis.

Authors:  Ming-Hsiun Hsieh; Chiung-Yun Chang; Shih-Jui Hsu; Ju-Jiun Chen
Journal:  Plant Mol Biol       Date:  2008-01-31       Impact factor: 4.076
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