| Literature DB >> 2003916 |
M W Allard1, D L Ellsworth, R L Honeycutt.
Abstract
A simple and reliable procedure for the amplification of single-stranded DNA suitable for sequencing is described. This procedure employs the polymerase chain reaction and implements modifications pertaining to the purification of the double-stranded DNA product prior to single-stranded DNA amplification. The most consistent sequencing reactions are obtained when the double-stranded DNA product is purified by centrifugation with a microconcentrator prior to single-stranded DNA amplification and the overall amount of specific primers and number of cycles used, in both single-stranded and double-stranded DNA polymerase chain reactions, are reduced.Mesh：
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Year: 1991 PMID： 2003916
Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993