OBJECTIVE: To establish chromatographic fingerprints of triterpenoid compounds in ganoderma RP-HPLC for the quality control of ganoderma. METHOD: HPLC fingerprints were established. The chromatographic conditions were as follows: the separation was performed on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with acetonitrile-water containing 0.8% perchloric acid as the mobile phases. The flow rate was 0.9 mL x min(-1), the detection wavelength was 254 nm and the temperature of column was at ambient temperature. The common patterns of HPTLC and HPLC fingerprints were separately obtained through "Chromafinger" solution software, and quality assessments were analyzed by similarity. RESULT: The HPLC fingerprint pattern of G. lucidum consists of 18 peaks, among them 6 peaks were identified by chemical reference substances. CONCLUSION: This study provided theory and experimental data in order to comprehensively evaluate the quality of ganoderma.
OBJECTIVE: To establish chromatographic fingerprints of triterpenoid compounds in ganoderma RP-HPLC for the quality control of ganoderma. METHOD: HPLC fingerprints were established. The chromatographic conditions were as follows: the separation was performed on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with acetonitrile-water containing 0.8% perchloric acid as the mobile phases. The flow rate was 0.9 mL x min(-1), the detection wavelength was 254 nm and the temperature of column was at ambient temperature. The common patterns of HPTLC and HPLC fingerprints were separately obtained through "Chromafinger" solution software, and quality assessments were analyzed by similarity. RESULT: The HPLC fingerprint pattern of G. lucidum consists of 18 peaks, among them 6 peaks were identified by chemical reference substances. CONCLUSION: This study provided theory and experimental data in order to comprehensively evaluate the quality of ganoderma.