| Literature DB >> 20026326 |
Rikako Sanuki1, Yoshihiro Omori, Chieko Koike, Shigeru Sato, Takahisa Furukawa.
Abstract
Neuronal gene transcription is regulated by both transcriptional activators and repressors. While the roles of transactivators in retinal photoreceptor development have been well characterized, the roles of repressors have been poorly understood. We isolated Panky/Ankrd33, a gene encoding an ankyrin repeat-containing protein. Panky-A was specifically expressed in retinal photoreceptors and the pineal gland, and its expression was directly up-regulated by the CRX transcription factor. Subcellular localization of PANKY-A was observed in the nucleus and cytoplasm. Additionally, transactivation analysis suggested that PANKY-A is a transcriptional cofactor that suppresses CRX-activated photoreceptor genes. Furthermore, we found by an electrophoretic mobility shift assay that PANKY inhibited the DNA-binding activity of CRX. Copyright 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.Entities:
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Year: 2009 PMID： 20026326 DOI： 10.1016/j.febslet.2009.12.030
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124