| Literature DB >> 20014789 |
Xiayan Wang1, Vijaykumar Veerappan, Chang Cheng, Xin Jiang, Randy D Allen, Purnendu K Dasgupta, Shaorong Liu.
Abstract
Gel electrophoresis is commonly used to separate DNA, but narrow capillaries or microchannels desired for high throughput efficient separations are difficult to fill with gels. We report here that a narrow capillary can be used to hydrodynamically separate a wide size range of DNA fragments in a single run without the need for gels, wall coatings, or an electric field. We also demonstrate that attractive separation is possible in a few minutes and that the separated DNA can be collected into individual fractions that remain viable for amplification via the polymerase chain reaction.Mesh：
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Year: 2010 PMID： 20014789 DOI： 10.1021/ja909233n
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419