Literature DB >> 20013786

Design, synthesis, and amplification of DNA pools for in vitro selection.

Bradley Hall1, John M Micheletti, Pooja Satya, Krystal Ogle, Jack Pollard, Andrew D Ellington.   

Abstract

Preparation of a random-sequence DNA pool is presented. The degree of randomization and the length of the random sequence are discussed, as is synthesis of the pool using a DNA synthesizer or via commercial synthesis companies. Purification of a single-stranded pool and conversion to a double-stranded pool are presented as step-by-step protocols. Support protocols describe determination of the complexity and skewing of the pool, and optimization of amplification conditions.

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Year:  2009        PMID： 20013786     DOI： 10.1002/0471142700.nc0902s39

Source DB:  PubMed          Journal:  Curr Protoc Nucleic Acid Chem        ISSN： 1934-9270


  10 in total

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2.  Probing the limits of aptamer affinity with a microfluidic SELEX platform.

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Journal:  PLoS One       Date:  2011-11-14       Impact factor: 3.240

Review 3.  Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications.

Authors:  Ka Lok Hong; Letha J Sooter
Journal:  Biomed Res Int       Date:  2015-06-23       Impact factor: 3.411

4.  Aptamer-targeted antigen delivery.

Authors:  Brian C Wengerter; Joseph A Katakowski; Jacob M Rosenberg; Chae Gyu Park; Steven C Almo; Deborah Palliser; Matthew Levy
Journal:  Mol Ther       Date:  2014-03-31       Impact factor: 11.454

Review 5.  Automation in the high-throughput selection of random combinatorial libraries--different approaches for select applications.

Authors:  Jörn Glökler; Tatjana Schütze; Zoltán Konthur
Journal:  Molecules       Date:  2010-04-08       Impact factor: 4.411

Review 6.  Inside the Black Box: What Makes SELEX Better?

Authors:  Natalia Komarova; Alexander Kuznetsov
Journal:  Molecules       Date:  2019-10-07       Impact factor: 4.411

Review 7.  Aptamers, the Nucleic Acid Antibodies, in Cancer Therapy.

Authors:  Zhaoying Fu; Jim Xiang
Journal:  Int J Mol Sci       Date:  2020-04-17       Impact factor: 5.923

8.  An RNA alternative to human transferrin: a new tool for targeting human cells.

Authors:  Samantha E Wilner; Brian Wengerter; Keith Maier; Maria de Lourdes Borba Magalhães; David Soriano Del Amo; Supriya Pai; Felipe Opazo; Silvio O Rizzoli; Amy Yan; Matthew Levy
Journal:  Mol Ther Nucleic Acids       Date:  2012-05-15       Impact factor: 10.183

Review 9.  RNA modularity for synthetic biology.

Authors:  Wade Grabow; Luc Jaeger
Journal:  F1000Prime Rep       Date:  2013-11-01

10.  Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga Toxins.

Authors:  Sreerupa Challa; Saul Tzipori; Abhineet Sheoran
Journal:  J Nucleic Acids       Date:  2014-04-15
  10 in total

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