Literature DB >> 20013755

Fluorescence correlation spectroscopy in living cells: a practical approach.

Nihal Altan-Bonnet1, Grégoire Altan-Bonnet.   

Abstract

Fluorescence correlation spectroscopy (FCS) is a single-molecule fluorescence technique used to monitor molecular dynamics. It can be applied to living cells expressing fluorescently labeled proteins and lipids to determine the diffusion timescales and the total number of diffusing fluorescent molecules in the cell. A practical step-by-step approach to performing FCS with a commercial spectroscopy/microscopy system, the Zeiss Confocor 3, how to set up live-cell FCS experiments, acquire reliable data, and finally how to analyze the data acquired, are all described in this unit. Copyright 2009 by John Wiley & Sons, Inc.

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Year:  2009        PMID： 20013755      PMCID： PMC3812671          DOI： 10.1002/0471143030.cb0424s45

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN： 1934-2616


  9 in total

Review 1.  Fluorescence correlation spectroscopy and its potential for intracellular applications.

Authors:  P Schwille
Journal:  Cell Biochem Biophys       Date:  2001       Impact factor: 2.194

2.  Bubble dynamics in double-stranded DNA.

Authors:  Grégoire Altan-Bonnet; Albert Libchaber; Oleg Krichevsky
Journal:  Phys Rev Lett       Date:  2003-04-01       Impact factor: 9.161

3.  Anomalous protein diffusion in living cells as seen by fluorescence correlation spectroscopy.

Authors:  Matthias Weiss; Hitoshi Hashimoto; Tommy Nilsson
Journal:  Biophys J       Date:  2003-06       Impact factor: 4.033

Review 4.  A dynamic view of cellular processes by in vivo fluorescence auto- and cross-correlation spectroscopy.

Authors:  Kirsten Bacia; Petra Schwille
Journal:  Methods       Date:  2003-01       Impact factor: 3.608

5.  Automatic real-time three-dimensional cell tracking by fluorescence microscopy.

Authors:  G Rabut; J Ellenberg
Journal:  J Microsc       Date:  2004-11       Impact factor: 1.758

Review 6.  Fluorescence correlation spectroscopy as a tool to investigate chemical reactions in solutions and on cell surfaces.

Authors:  J Widengren; R Rigler
Journal:  Cell Mol Biol (Noisy-le-grand)       Date:  1998-07       Impact factor: 1.770

7.  Kinetics of conformational fluctuations in DNA hairpin-loops.

Authors:  G Bonnet; O Krichevsky; A Libchaber
Journal:  Proc Natl Acad Sci U S A       Date:  1998-07-21       Impact factor: 11.205

8.  Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy.

Authors:  U Haupts; S Maiti; P Schwille; W W Webb
Journal:  Proc Natl Acad Sci U S A       Date:  1998-11-10       Impact factor: 11.205

9.  Golgi inheritance in mammalian cells is mediated through endoplasmic reticulum export activities.

Authors:  Nihal Altan-Bonnet; Rachid Sougrat; Wei Liu; Erik L Snapp; Theresa Ward; Jennifer Lippincott-Schwartz
Journal:  Mol Biol Cell       Date:  2005-11-28       Impact factor: 4.138
  9 in total
  7 in total

1.  Measuring protein dynamics in live cells: protocols and practical considerations for fluorescence fluctuation microscopy.

Authors:  Robert T Youker; Haibing Teng
Journal:  J Biomed Opt       Date:  2014-09       Impact factor: 3.170

2.  3D Protein Dynamics in the Cell Nucleus.

Authors:  Anand P Singh; Rémi Galland; Megan L Finch-Edmondson; Gianluca Grenci; Jean-Baptiste Sibarita; Vincent Studer; Virgile Viasnoff; Timothy E Saunders
Journal:  Biophys J       Date:  2017-01-10       Impact factor: 4.033

3.  Dopamine Receptor Signaling in MIN6 β-Cells Revealed by Fluorescence Fluctuation Spectroscopy.

Authors:  Brittany Caldwell; Alessandro Ustione; David W Piston
Journal:  Biophys J       Date:  2016-08-09       Impact factor: 4.033

4.  Quantitative Control of GPCR Organization and Signaling by Endocytosis in Epithelial Morphogenesis.

Authors:  Ankita Jha; Thomas S van Zanten; Jean-Marc Philippe; Satyajit Mayor; Thomas Lecuit
Journal:  Curr Biol       Date:  2018-05-03       Impact factor: 10.834

5.  Distinct actin-dependent nanoscale assemblies underlie the dynamic and hierarchical organization of E-cadherin.

Authors:  Rumamol Chandran; Girish Kale; Jean-Marc Philippe; Thomas Lecuit; Satyajit Mayor
Journal:  Curr Biol       Date:  2021-02-18       Impact factor: 10.834

6.  Nanomolar Nitric Oxide Concentrations in Living Cells Measured by Means of Fluorescence Correlation Spectroscopy.

Authors:  Roksana Markiewicz; Jagoda Litowczenko; Jacek Gapiński; Anna Woźniak; Stefan Jurga; Adam Patkowski
Journal:  Molecules       Date:  2022-02-02       Impact factor: 4.411

7.  Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells.

Authors:  Christian H Gabriel; Marta Del Olmo; Amin Zehtabian; Marten Jäger; Silke Reischl; Hannah van Dijk; Carolin Ulbricht; Asylkhan Rakhymzhan; Thomas Korte; Barbara Koller; Astrid Grudziecki; Bert Maier; Andreas Herrmann; Raluca Niesner; Tomasz Zemojtel; Helge Ewers; Adrián E Granada; Hanspeter Herzel; Achim Kramer
Journal:  Nat Commun       Date:  2021-06-18       Impact factor: 14.919
  7 in total

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