| Literature DB >> 20013232 |
Ruben van Boxtel1, Michael N Gould, Edwin Cuppen, Bart M G Smits.
Abstract
The rat is one of the most preferred model organisms in biomedical research and has been extremely useful for linking physiology and pathology to the genome. However, approaches to genetically modify specific genes in the rat germ line remain relatively scarce. To date, the most efficient approach for generating genetically modified rats has been the target-selected N-ethyl-N-nitrosourea (ENU) mutagenesis-based technology. Here, we describe the detailed protocols for ENU mutagenesis and mutant retrieval in the rat model organism.Entities:
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Year: 2010 PMID： 20013232 DOI： 10.1007/978-1-60327-389-3_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745