BACKGROUND: Psoriasis and atopic dermatitis (AD) are common, complex inflammatory skin diseases. Both diseases display immune infiltrates in lesions and epidermal growth/differentiation alterations associated with a defective skin barrier. An incomplete understanding of differences between these diseases makes it difficult to compare human disease pathology to animal disease models. OBJECTIVE: To characterize differences between these diseases in expression of genes related to epidermal growth/differentiation and inflammatory circuits. METHODS: We performed genomic profiling of mRNA in chronic psoriasis (n = 15) and AD (n = 18) skin lesions compared with normal human skin (n = 15). RESULTS: As expected, clear disease classifications could be constructed on the basis of expected immune polarity (T(H)1, T(H)2, T(H)17) differences. However, even more striking differences were identified in epidermal differentiation programs that could be used for precise disease classifications. Although both psoriasis and AD skin lesions displayed regenerative epidermal hyperplasia, which is a general alteration in epidermal growth, keratinocyte terminal differentiation was differentially polarized. In AD, we found selective defects in expression of multiple genes encoding the cornified envelope, with the largest alteration in loricrin (expressed at 2% of the level of normal skin). At the ultrastructural level, the cornified envelope in AD was broadly defective with highly decreased compaction of corneocytes and reduced intercellular lipids. Hence, the entire keratinocyte terminal differentiation program (cytoplasmic compaction, cornification, and lipid release) is defective in AD, potentially underlying the immune differences. CONCLUSION: Our study shows that although alterations in barrier responses exist in both diseases, epidermal differentiation is differentially polarized, with major implications for primary disease pathogenesis.
BACKGROUND:Psoriasis and atopic dermatitis (AD) are common, complex inflammatory skin diseases. Both diseases display immune infiltrates in lesions and epidermal growth/differentiation alterations associated with a defective skin barrier. An incomplete understanding of differences between these diseases makes it difficult to compare human disease pathology to animal disease models. OBJECTIVE: To characterize differences between these diseases in expression of genes related to epidermal growth/differentiation and inflammatory circuits. METHODS: We performed genomic profiling of mRNA in chronic psoriasis (n = 15) and AD (n = 18) skin lesions compared with normal human skin (n = 15). RESULTS: As expected, clear disease classifications could be constructed on the basis of expected immune polarity (T(H)1, T(H)2, T(H)17) differences. However, even more striking differences were identified in epidermal differentiation programs that could be used for precise disease classifications. Although both psoriasis and AD skin lesions displayed regenerative epidermal hyperplasia, which is a general alteration in epidermal growth, keratinocyte terminal differentiation was differentially polarized. In AD, we found selective defects in expression of multiple genes encoding the cornified envelope, with the largest alteration in loricrin (expressed at 2% of the level of normal skin). At the ultrastructural level, the cornified envelope in AD was broadly defective with highly decreased compaction of corneocytes and reduced intercellular lipids. Hence, the entire keratinocyte terminal differentiation program (cytoplasmic compaction, cornification, and lipid release) is defective in AD, potentially underlying the immune differences. CONCLUSION: Our study shows that although alterations in barrier responses exist in both diseases, epidermal differentiation is differentially polarized, with major implications for primary disease pathogenesis.
Authors: Carrie Hayes Sutter; Sridevi Bodreddigari; Christina Campion; Ryan S Wible; Thomas R Sutter Journal: Toxicol Sci Date: 2011-08-11 Impact factor: 4.849
Authors: Hitokazu Esaki; David A Ewald; Benjamin Ungar; Mariya Rozenblit; Xiuzhong Zheng; Hui Xu; Yeriel D Estrada; Xiangyu Peng; Hiroshi Mitsui; Thomas Litman; Mayte Suárez-Fariñas; James G Krueger; Emma Guttman-Yassky Journal: J Allergy Clin Immunol Date: 2015-01 Impact factor: 10.793
Authors: Joonsung Hwang; Ryosuke Kita; Hyouk-Soo Kwon; Eung Ho Choi; Seung Hun Lee; Mark C Udey; Maria I Morasso Journal: Proc Natl Acad Sci U S A Date: 2011-06-27 Impact factor: 11.205