AIM: To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 (TRPM7) in the human gastrointestinal (GI) tract. METHODS: Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajal (ICCs). RESULTS: The human GI tract generated slow electrical waves and had ICCs which functioned as pacemaker cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB (2-aminoethoxydiphenyl borate) and La(3+), TRPM7 channel blockers, inhibited the slow waves. Also, TRPM7 channels were expressed in ICCs in human tissue. CONCLUSION: These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the generation of the slow waves.
AIM: To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 (TRPM7) in the human gastrointestinal (GI) tract. METHODS: Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajal (ICCs). RESULTS: The human GI tract generated slow electrical waves and had ICCs which functioned as pacemaker cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB (2-aminoethoxydiphenyl borate) and La(3+), TRPM7 channel blockers, inhibited the slow waves. Also, TRPM7 channels were expressed in ICCs in human tissue. CONCLUSION: These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the generation of the slow waves.
Authors: Carsten Schmitz; Anne-Laure Perraud; Catherine O Johnson; Kazunori Inabe; Megan K Smith; Reinhold Penner; Tomohiro Kurosaki; Andrea Fleig; Andrew M Scharenberg Journal: Cell Date: 2003-07-25 Impact factor: 41.582
Authors: Peter R Strege; Yijun Ou; Lei Sha; Adam Rich; Simon J Gibbons; Joseph H Szurszewski; Michael G Sarr; Gianrico Farrugia Journal: Am J Physiol Gastrointest Liver Physiol Date: 2003-07-31 Impact factor: 4.052
Authors: Michelle Aarts; Koji Iihara; Wen-Li Wei; Zhi-Gang Xiong; Mark Arundine; Waldy Cerwinski; John F MacDonald; Michael Tymianski Journal: Cell Date: 2003-12-26 Impact factor: 41.582
Authors: Mahealani K Monteilh-Zoller; Meredith C Hermosura; Monica J S Nadler; Andrew M Scharenberg; Reinhold Penner; Andrea Fleig Journal: J Gen Physiol Date: 2003-01 Impact factor: 4.086