| Literature DB >> 19997890 |
Victoria J Spanswick1, Janet M Hartley, John A Hartley.
Abstract
The Single Cell Gel Electrophoresis (Comet) assay, originally developed to allow visualisation of DNA strand break damage in individual cells, has been adapted to measure DNA interstrand cross-links. DNA interstrand cross-links are formed in cells by a number of commonly used cancer chemotherapy agents and are considered to be the critical lesion formed by such agents. This technique allows the analysis of DNA interstrand cross-link formation and repair at a single cell level, requires few cells, allows the determination of heterogeneity of response within a cell population and is sensitive enough to measure DNA interstrand cross-links at pharmacologically relevant doses. The method can be applied to any in vitro or in vivo application where a single cell suspension can be obtained. The method has also become invaluable in studies using human tissue and can be used as a method for pharmacodynamic analysis in early clinical trials.Entities:
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Year: 2010 PMID： 19997890 DOI： 10.1007/978-1-60327-418-0_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745