Literature DB >> 19948854

Use of green fluorescent protein to monitor cell envelope stress in Lactococcus lactis.

Ana Belén Campelo1, Ana Rodríguez, Beatriz Martínez.   

Abstract

A Lactococcus lactis reporter system suitable to detect cell envelope stress in high-throughput settings was developed by fusing the CesR-regulated promoter of llmg0169 to the gfp(uv) gene. A dot blot assay allowed fast detection of green fluorescent protein (GFP) fluorescence even at low production levels. Unexpectedly, this promoter was also induced by mitomycin C via CesR.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19948854      PMCID： PMC2813010          DOI： 10.1128/AEM.02177-09

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  17 in total

1.  Optimization of fed-batch production of the model recombinant protein GFP in Lactococcus lactis.

Authors:  Gian M Oddone; Christopher Q Lan; Helen Rawsthorne; David A Mills; David E Block
Journal:  Biotechnol Bioeng       Date:  2007-04-15       Impact factor: 4.530

2.  Protein activity in bacterial inclusion bodies correlates with predicted aggregation rates.

Authors:  Natalia Sánchez de Groot; Salvador Ventura
Journal:  J Biotechnol       Date:  2006-04-18       Impact factor: 3.307

3.  Validation for high-throughput screening of a VanRS-based reporter gene assay for bacterial cell wall inhibitors.

Authors:  G De Pascale; C Grigoriadou; D Losi; I Ciciliato; M Sosio; S Donadio
Journal:  J Appl Microbiol       Date:  2007-07       Impact factor: 3.772

4.  Bioassay for nisin in milk, processed cheese, salad dressings, canned tomatoes, and liquid egg products.

Authors:  J Hakovirta; J Reunanen; P E J Saris
Journal:  Appl Environ Microbiol       Date:  2006-02       Impact factor: 4.792

5.  Multicopy integration of heterologous genes, using the lactococcal group II intron targeted to bacterial insertion sequences.

Authors:  Helen Rawsthorne; Kevin N Turner; David A Mills
Journal:  Appl Environ Microbiol       Date:  2006-09       Impact factor: 4.792

6.  Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis.

Authors:  D Simon; A Chopin
Journal:  Biochimie       Date:  1988-04       Impact factor: 4.079

7.  Incorporation of nisI-mediated nisin immunity improves vector-based nisin-controlled gene expression in lactic acid bacteria.

Authors:  Gian M Oddone; David A Mills; David E Block
Journal:  Plasmid       Date:  2009-01-12       Impact factor: 3.466

8.  Cell envelope stress induced by the bacteriocin Lcn972 is sensed by the Lactococcal two-component system CesSR.

Authors:  Beatriz Martínez; Aldert L Zomer; Ana Rodríguez; Jan Kok; Oscar P Kuipers
Journal:  Mol Microbiol       Date:  2007-04       Impact factor: 3.501

9.  Use of the Escherichia coli beta-glucuronidase (gusA) gene as a reporter gene for analyzing promoters in lactic acid bacteria.

Authors:  C Platteeuw; G Simons; W M de Vos
Journal:  Appl Environ Microbiol       Date:  1994-02       Impact factor: 4.792

10.  Contribution of the CesR-regulated genes llmg0169 and llmg2164-2163 to Lactococcus lactis fitness.

Authors:  Clara Roces; Ana B Campelo; Patrick Veiga; João P C Pinto; Ana Rodríguez; Beatriz Martínez
Journal:  Int J Food Microbiol       Date:  2009-06-07       Impact factor: 5.277
View more
  2 in total

1.  Cell Wall-active Bacteriocins and Their Applications Beyond Antibiotic Activity.

Authors:  Clara Roces; Ana Rodríguez; Beatriz Martínez
Journal:  Probiotics Antimicrob Proteins       Date:  2012-12       Impact factor: 4.609

2.  Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging.

Authors:  Wout Overkamp; Katrin Beilharz; Ruud Detert Oude Weme; Ana Solopova; Harma Karsens; Ákos T Kovács; Jan Kok; Oscar P Kuipers; Jan-Willem Veening
Journal:  Appl Environ Microbiol       Date:  2013-08-16       Impact factor: 4.792
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.