OBJECT: Glial cell line-derived neurotrophic factor (GDNF) has potent survival effects on central and peripheral nerve populations. The authors examined the differential effects of GDNF following either a sciatic nerve crush injury in mice that overexpressed GDNF in the central or peripheral nervous systems (glial fibrillary acidic protein [GFAP]-GDNF) or in the muscle target (Myo-GDNF). METHODS: Adult mice (GFAP-GDNF, Myo-GDNF, or wild-type [WT] animals) underwent sciatic nerve crush and were evaluated using histomorphometry and muscle force and power testing. Uninjured WT animals served as controls. RESULTS: In the sciatic nerve crush, the Myo-GDNF mice demonstrated a higher number of nerve fibers, fiber density, and nerve percentage (p < 0.05) at 2 weeks. The early regenerative response did not result in superlative functional recovery. At 3 weeks, GFAP-GDNF animals exhibit fewer nerve fibers, decreased fiber width, and decreased nerve percentage compared with WT and Myo-GDNF mice (p < 0.05). By 6 weeks, there were no significant differences between groups. CONCLUSIONS: Peripheral delivery of GDNF resulted in earlier regeneration following sciatic nerve crush injuries than that with central GDNF delivery. Treatment with neurotrophic factors such as GDNF may offer new possibilities for the treatment of peripheral nerve injury.
OBJECT: Glial cell line-derived neurotrophic factor (GDNF) has potent survival effects on central and peripheral nerve populations. The authors examined the differential effects of GDNF following either a sciatic nerve crush injury in mice that overexpressed GDNF in the central or peripheral nervous systems (glial fibrillary acidic protein [GFAP]-GDNF) or in the muscle target (Myo-GDNF). METHODS: Adult mice (GFAP-GDNF, Myo-GDNF, or wild-type [WT] animals) underwent sciatic nerve crush and were evaluated using histomorphometry and muscle force and power testing. Uninjured WT animals served as controls. RESULTS: In the sciatic nerve crush, the Myo-GDNFmice demonstrated a higher number of nerve fibers, fiber density, and nerve percentage (p < 0.05) at 2 weeks. The early regenerative response did not result in superlative functional recovery. At 3 weeks, GFAP-GDNF animals exhibit fewer nerve fibers, decreased fiber width, and decreased nerve percentage compared with WT and Myo-GDNFmice (p < 0.05). By 6 weeks, there were no significant differences between groups. CONCLUSIONS: Peripheral delivery of GDNF resulted in earlier regeneration following sciatic nerve crush injuries than that with central GDNF delivery. Treatment with neurotrophic factors such as GDNF may offer new possibilities for the treatment of peripheral nerve injury.
Authors: C E Henderson; H S Phillips; R A Pollock; A M Davies; C Lemeulle; M Armanini; L Simmons; B Moffet; R A Vandlen; L Simpson LC corrected to Simmons; V E Koliatsos; A Rosenthal Journal: Science Date: 1994-11-11 Impact factor: 47.728
Authors: R W Oppenheim; L J Houenou; J E Johnson; L F Lin; L Li; A C Lo; A L Newsome; D M Prevette; S Wang Journal: Nature Date: 1995-01-26 Impact factor: 49.962
Authors: Ruben Eggers; Fred de Winter; Stefan A Hoyng; Kasper C D Roet; Erich M Ehlert; Martijn J A Malessy; Joost Verhaagen; Martijn R Tannemaat Journal: PLoS One Date: 2013-08-12 Impact factor: 3.240
Authors: Kristian L Hedstrom; Joshua C Murtie; Kathryn Albers; Nigel A Calcutt; Gabriel Corfas Journal: Proc Natl Acad Sci U S A Date: 2014-01-21 Impact factor: 11.205
Authors: Nesreen Zoghoul Alsmadi; Geetanjali S Bendale; Aswini Kanneganti; Tarik Shihabeddin; An H Nguyen; Elijah Hor; Swarup Dash; Benjamin Johnston; Rafael Granja-Vazquez; Mario I Romero-Ortega Journal: Acta Biomater Date: 2018-07-29 Impact factor: 8.947
Authors: Yuewei Wu-Fienberg; Amy M Moore; Laura M Marquardt; Piyaraj Newton; Philip J Johnson; Susan E Mackinnon; Shelly E Sakiyama-Elbert; Matthew D Wood Journal: Biotechnol Bioeng Date: 2014-04-24 Impact factor: 4.530
Authors: Bogdan Beirowski; Jason Gustin; Sean M Armour; Hiroyasu Yamamoto; Andreu Viader; Brian J North; Shaday Michán; Robert H Baloh; Judy P Golden; Robert E Schmidt; David A Sinclair; Johan Auwerx; Jeffrey Milbrandt Journal: Proc Natl Acad Sci U S A Date: 2011-09-26 Impact factor: 11.205