| Literature DB >> 19941831 |
John R Sheedy1, Peter R Ebeling, Paul R Gooley, Malcolm J McConville.
Abstract
We describe a general protocol for preparing protein-containing biofluids for (1)H nuclear magnetic resonance (NMR) metabolomic studies. In this protocol, untreated samples are diluted in deuterated solvents to precipitate proteins and recover metabolites quantitated relative to standard reference compounds such as 3-trimethylsilylpropionic acid (TSP) and 2,2-dimethyl-2-silapentane-5-sulfonic acid (DSS). The efficacy of this protocol was tested using a bovine serum albumin/metabolite mix and human serum samples. This sample preparation method can be readily applied to any protein-containing biofluid for (1)H NMR studies. Copyright (c) 2009 Elsevier Inc. All rights reserved.Entities:
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Year: 2009 PMID： 19941831 DOI： 10.1016/j.ab.2009.11.027
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365