Preparation of synthetic polyoxazoline based carrier and Vibrio cholerae O-specific polysaccharide conjugate vaccine.

Multiple chemical attachments of carbohydrate antigens to linear polymer represent promising technique for creating biologically effective conjugates. A novel conjugate consisting of detoxified lipopolysaccharide of Vibrio cholerae O135, linear polymer (polyoxazoline copolymer, serving as a matrix) and BSA (as immunogenic protein), has been prepared. The reaction conditions were optimized for obtaining high degree of conjugation. Analytical methods were evaluated to characterize conjugates obtained. Proposed chemistry is suitable for preparation of multivalent glycoconjugates in general. Copyright 2009 Elsevier Masson SAS. All rights reserved.