Literature DB >> 19937720

Investigating solution-phase protein structure and dynamics by hydrogen exchange mass spectrometry.

Christopher R Morgan1, John R Engen.   

Abstract

By taking advantage of labeling methods such as hydrogen exchange (HX), many details about protein conformation, dynamics, and interactions can be revealed by mass spectrometry. In this unit, hydrogen exchange theory is discussed as it applies to HX-MS protocols, the practice of HX-MS including data analysis and interpretation is explained in detail, and recent advancements in technology which greatly increase the depth of information gained from the technique are highlighted.

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Year:  2009        PMID： 19937720      PMCID： PMC2844867          DOI： 10.1002/0471140864.ps1706s58

Source DB:  PubMed          Journal:  Curr Protoc Protein Sci        ISSN： 1934-3655


  55 in total

Review 1.  The hydrogen exchange core and protein folding.

Authors:  R Li; C Woodward
Journal:  Protein Sci       Date:  1999-08       Impact factor: 6.725

Review 2.  Protein analysis by hydrogen exchange mass spectrometry.

Authors:  Andrew N Hoofnagle; Katheryn A Resing; Natalie G Ahn
Journal:  Annu Rev Biophys Biomol Struct       Date:  2003-02-18

3.  Mass spectrometric determination of isotopic exchange rates of amide hydrogens located on the surfaces of proteins.

Authors:  K Dharmasiri; D L Smith
Journal:  Anal Chem       Date:  1996-07-15       Impact factor: 6.986

4.  Identification of unfolding domains in large proteins by their unfolding rates.

Authors:  Y Deng; D L Smith
Journal:  Biochemistry       Date:  1998-05-05       Impact factor: 3.162

5.  Probing the non-covalent structure of proteins by amide hydrogen exchange and mass spectrometry.

Authors:  D L Smith; Y Deng; Z Zhang
Journal:  J Mass Spectrom       Date:  1997-02       Impact factor: 1.982

6.  Effect of irreversibility on the thermodynamic characterization of the thermal denaturation of Aspergillus saitoi acid proteinase.

Authors:  S R Tello-Solis; A Hernandez-Arana
Journal:  Biochem J       Date:  1995-11-01       Impact factor: 3.857

7.  Comparison of continuous and pulsed labeling amide hydrogen exchange/mass spectrometry for studies of protein dynamics.

Authors:  Y Deng; Z Zhang; D L Smith
Journal:  J Am Soc Mass Spectrom       Date:  1999-08       Impact factor: 3.109

8.  Kinetics of cytochrome c folding examined by hydrogen exchange and mass spectrometry.

Authors:  H Yang; D L Smith
Journal:  Biochemistry       Date:  1997-12-02       Impact factor: 3.162

9.  Identification and localization of slow, natural, cooperative unfolding in the hematopoietic cell kinase SH3 domain by amide hydrogen exchange and mass spectrometry.

Authors:  J R Engen; T E Smithgall; W H Gmeiner; D L Smith
Journal:  Biochemistry       Date:  1997-11-25       Impact factor: 3.162

10.  Measurement of amide hydrogen exchange by MALDI-TOF mass spectrometry.

Authors:  J G Mandell; A M Falick; E A Komives
Journal:  Anal Chem       Date:  1998-10-01       Impact factor: 6.986
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  32 in total

1.  Solution structure, conformational dynamics, and CD4-induced activation in full-length, glycosylated, monomeric HIV gp120.

Authors:  Miklos Guttman; Maria Kahn; Natalie K Garcia; Shiu-Lok Hu; Kelly K Lee
Journal:  J Virol       Date:  2012-06-06       Impact factor: 5.103

2.  Molecular insight into the conformational dynamics of the Elongin BC complex and its interaction with HIV-1 Vif.

Authors:  Sean R Marcsisin; John R Engen
Journal:  J Mol Biol       Date:  2010-08-20       Impact factor: 5.469

3.  Identification of a Structural Determinant for Selective Targeting of HDMX.

Authors:  Yael Ben-Nun; Hyuk-Soo Seo; Edward P Harvey; Zachary J Hauseman; Thomas E Wales; Catherine E Newman; Ann M Cathcart; John R Engen; Sirano Dhe-Paganon; Loren D Walensky
Journal:  Structure       Date:  2020-04-30       Impact factor: 5.006

4.  The utility of hydrogen/deuterium exchange mass spectrometry in biopharmaceutical comparability studies.

Authors:  Damian Houde; Steven A Berkowitz; John R Engen
Journal:  J Pharm Sci       Date:  2010-12-29       Impact factor: 3.534

5.  Calcium-induced structural rearrangements release autoinhibition in the Rap-GEF CalDAG-GEFI.

Authors:  Aaron A Cook; Wei Deng; Jinqi Ren; Renhao Li; John Sondek; Wolfgang Bergmeier
Journal:  J Biol Chem       Date:  2018-04-05       Impact factor: 5.157

6.  Caspase-6 Undergoes a Distinct Helix-Strand Interconversion upon Substrate Binding.

Authors:  Kevin B Dagbay; Nicolas Bolik-Coulon; Sergey N Savinov; Jeanne A Hardy
Journal:  J Biol Chem       Date:  2017-02-02       Impact factor: 5.157

7.  Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics.

Authors:  Cristina Lento; Shaolong Zhu; Kerene A Brown; Ruth Knox; Peter Liuni; Derek J Wilson
Journal:  J Vis Exp       Date:  2017-04-17       Impact factor: 1.355

8.  Multiple proteolytic events in caspase-6 self-activation impact conformations of discrete structural regions.

Authors:  Kevin B Dagbay; Jeanne A Hardy
Journal:  Proc Natl Acad Sci U S A       Date:  2017-09-01       Impact factor: 11.205

9.  Monitoring protein conformational changes and dynamics using stable-isotope labeling and mass spectrometry.

Authors:  Alem W Kahsai; Sudarshan Rajagopal; Jinpeng Sun; Kunhong Xiao
Journal:  Nat Protoc       Date:  2014-05-08       Impact factor: 13.491

10.  HDX-MS reveals orthosteric and allosteric changes in apolipoprotein-D structural dynamics upon binding of progesterone.

Authors:  Claudia S Kielkopf; Madhubrata Ghosh; Ganesh S Anand; Simon H J Brown
Journal:  Protein Sci       Date:  2018-12-20       Impact factor: 6.725
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