OBJECTIVES: To determine the prevalence and the diversity of extended-spectrum beta-lactamases (ESBLs) among Escherichia coli isolates in Zhengzhou, China. METHODS: Clinical isolates were collected and investigated from the first affiliated hospital of Zhengzhou University and its associated health-care facilities in Zhengzhou, China, during the period from January 2006 to June 2008. Antibiograms were performed on Mueller-Hinton agar plates with the disc-diffusion method and MICs were determined by the agar-dilution method. Total DNA was extracted with a Qiagen mini kit and screened by PCR. RESULTS: Of 94 nonduplicate ESBL-positive isolates, TEM-type was encoded in 74 and 79% of the ESBL isolates. Fifty-six isolates were SHV type ESBLs. CTX-M-1, CTX-M-14, CTX-M-25, and CTX-M-38 types were encoded in 30, 54, 6, and 4, respectively. OXA-1-type beta-lactamases were encoded in six and OXA-20-type was encoded in two isolates. CONCLUSIONS: We describe a complex ESBL epidemiology. The study revealed a high rate of ESBL-producing E. coli isolates. TEM and CTX-M enzymes dominated in ESBL-positive E. coli isolates in Zhengzhou, China.
OBJECTIVES: To determine the prevalence and the diversity of extended-spectrum beta-lactamases (ESBLs) among Escherichia coli isolates in Zhengzhou, China. METHODS: Clinical isolates were collected and investigated from the first affiliated hospital of Zhengzhou University and its associated health-care facilities in Zhengzhou, China, during the period from January 2006 to June 2008. Antibiograms were performed on Mueller-Hinton agar plates with the disc-diffusion method and MICs were determined by the agar-dilution method. Total DNA was extracted with a Qiagen mini kit and screened by PCR. RESULTS: Of 94 nonduplicate ESBL-positive isolates, TEM-type was encoded in 74 and 79% of the ESBL isolates. Fifty-six isolates were SHV type ESBLs. CTX-M-1, CTX-M-14, CTX-M-25, and CTX-M-38 types were encoded in 30, 54, 6, and 4, respectively. OXA-1-type beta-lactamases were encoded in six and OXA-20-type was encoded in two isolates. CONCLUSIONS: We describe a complex ESBL epidemiology. The study revealed a high rate of ESBL-producing E. coli isolates. TEM and CTX-M enzymes dominated in ESBL-positive E. coli isolates in Zhengzhou, China.
Authors: M Gniadkowski; I Schneider; A Pałucha; R Jungwirth; B Mikiewicz; A Bauernfeind Journal: Antimicrob Agents Chemother Date: 1998-04 Impact factor: 5.191
Authors: M Lavollay; K Mamlouk; T Frank; A Akpabie; B Burghoffer; S Ben Redjeb; R Bercion; V Gautier; G Arlet Journal: Antimicrob Agents Chemother Date: 2006-07 Impact factor: 5.191
Authors: R Bonnet; C Dutour; J L Sampaio; C Chanal; D Sirot; R Labia; C De Champs; J Sirot Journal: Antimicrob Agents Chemother Date: 2001-08 Impact factor: 5.191
Authors: N Woodford; M E Ward; M E Kaufmann; J Turton; E J Fagan; D James; A P Johnson; R Pike; M Warner; T Cheasty; A Pearson; S Harry; J B Leach; A Loughrey; J A Lowes; R E Warren; D M Livermore Journal: J Antimicrob Chemother Date: 2004-09-03 Impact factor: 5.790